Invitrogen™

pHrodo™ iFL Red STP Ester (amine-reactive)

Catalog number:  P36010

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Description

The amine-reactive pH-sensitive pHrodo iFL Red STP Ester dye is an improved version of pHrodo Red dye optimized for the creation of bioconjugates to be used in the study of antibody internalization, endocytosis, and phagocytosis. pHrodo iFL Red dye dramatically increases fluorescence as the pH of its surroundings become more acidic. It is more soluble than the original pHrodo Red dye, making it useful for the labeling of antibodies that may otherwise precipitate out of solution during conjugation.

• Use pH-sensitive pHrodo iFL Red STP Ester to make pH-sensitive bioconjugates of your choice
• Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers
• Multiplex with green fluorescent markers such as GFP, pHrodo iFL Green STP, and many others

The increase in fluorescence of pHrodo iFL Red dye as the pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it a valuable tool for the study of endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo iFL Red dye make it useful for multi-color experiments. pHrodo iFL Red dye can be detected using most standard red-fluorescent filter sets and has been validated for use in a variety of applications, including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo iFL Red dye in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.

pHrodo iFL Red STP Ester is an amine reactive dye that can be used to make pHrodo iFL Red STP bioconjugates in aqueous buffer. The STP ester will react with primary amines on a protein, cell, or virus to create a stable conjugate that can be used in live cell assays or stored for later use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity: Amine
Product Line: pHrodo™
Product Size: 1 mg
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

Documents

Manuals & protocols