Invitrogen™

pHrodo™ iFL Green Microscale Protein Labeling Kit

Catalog number: P36015
Invitrogen™

pHrodo™ iFL Green Microscale Protein Labeling Kit

Catalog number: P36015
Catalog Number
P36015
Unit Size
3 labelings
Price (USD)
Price: 654.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
P360153 labelings
Price: 654.00
Your Price:
-
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The pHrodo iFL Green Microscale Protein Labeling Kit provides the reagents needed to easily label 3 microscale purified protein or antibody samples (20–100 µg) with pHrodo iFL Green dye.

The pHrodo iFL Green Microscale Labeling Kit is:
Convenient—labeling typically takes 2 hours with less than 30 min hands-on time
Versatile—label proteins with molecular weights between 12 and 150 kDa
Customizable—create your own probes for studying antibody internalization, phagocytosis, and endocytosis

This kit contains amine-reactive pH-sensitive pHrodo iFL Green STP ester dye that covalently binds to free lysines anywhere they occur in an antibody or protein. pHrodo iFL Green dye is an improved version of pHrodoGreen dye optimized for the creation of bright and soluble bioconjugates to be used in the study of antibody internalization, endocytosis, and phagocytosis. It is more soluble than the original pHrodoGreen dye, making it more useful for the labeling of antibodies that may otherwise precipitate out of solution during conjugation. pHrodo iFL Green dye dramatically increases fluorescence as the pH of its surroundings become more acidic. It is non-fluorescent outside the cell, but fluoresces bright red in phagosomes and endosomes.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Protein Labeling Kit
Excitation/Emission
505/525
Labeling Scale
20 to 100 μg
Shipping Condition
Room Temperature
Product Line
pHrodo™
Label or Dye
pHrodo™ Green
Color
Green
Labeling Target
Antibodies (General), Proteins (General)

Contents & Storage

Store in refrigerator 2°C to 8°C, desiccate, protect from light, and do not freeze.