ProLong™ Gold Antifade Mountant with DNA Stains DAPI, 2 mL - FAQs

View additional product information for ProLong™ Gold Antifade Mountant with DNA Stain DAPI - FAQs (P36935, P36941, P36931)

7 product FAQs found

What do ProLong Gold, ProLong Diamond and ProLong Glass antifade mountant reagents contain?

They are composed of proprietary antifades and polymers in aqueous buffers.

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Can I use ProLong Gold, ProLong Diamond, or ProLong Glass mounting medium in a multi-well plate?

No. If the sample cannot be sealed with a coverslip, we recommend the use of SlowFade antifade mountant.

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What can affect sample curing of ProLong Gold-, ProLong Diamond-, and ProLong Glass-mounted samples?

Our ProLong Gold, ProLong Diamond, and ProLong Glass mounting media harden ('cure') by the evaporation of water. Placing the mounted samples in a humid or refrigerated environment would slow down the evaporation process ('curing').

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Are ProLong, ProLong Gold, ProLong Diamond, and ProLong Glass reagents compatible with DyLight dye-conjugated antibodies and streptavidin and with other organic dyes?

We have not tested ProLong, ProLong Gold, ProLong Diamond, or ProLong Glass on DyLight dye-conjugated proteins or with other organic dyes, but it is likely that they will be compatible.

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Can I use any of the ProLong, ProLong Gold, ProLong Diamond, or ProLong Glass reagents on live samples?

No. ProLong, ProLong Gold, ProLong Diamond, and ProLong Glass reagents are intended for use on fixed or fixed/permeabilized samples. Only ProLong Live Antifade Reagent (Cat Nos. P36974 and P36975) is intended for use with live cells.

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What happens when ProLong, ProLong Gold, ProLong Diamond, or ProLong Glass antifade mountant is curing? Is there a chemical reaction?

The evaporation of water is the “curing” process for Prolong, ProLong Gold, ProLong Diamond, and ProLong Glass antifade mountant. The evaporation of water causes the polymer to harden. There is no chemical reaction. Please note that there is no curing/hardening for any of the SlowFade reagents or ProLong Live products.

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DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

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