ProLong™ Diamond Antifade Mountant with DAPI
ProLong™ Diamond Antifade Mountant with DAPI
ProLong™ Diamond Antifade Mountant with DAPI
ProLong™ Diamond Antifade Mountant with DAPI
Invitrogen™

ProLong™ Diamond Antifade Mountant with DAPI

ProLong Diamond Antifade Mountant with DAPI is a glycerol-based liquid mountant designed to provide unparalleled antifade protection for fluorescence acrossRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
P369625 x 2 mL
P369662 mL
P3697110 mL
Catalog number P36962
Price (USD)
312.65
Online Exclusive
337.00
Save 24.35 (7%)
Each
Add to cart
Quantity:
5 x 2 mL
Price (USD)
312.65
Online Exclusive
337.00
Save 24.35 (7%)
Each
Add to cart

ProLong Diamond Antifade Mountant with DAPI is a glycerol-based liquid mountant designed to provide unparalleled antifade protection for fluorescence across the entire visual and IR spectrums. You can use ProLong Diamond Mountant with DAPI with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background. Use ProLong Diamond for bright, high-definition fluorescent images with DAPI already embedded within the mounting media for nuclear counterstains. ProLong Diamond mountant with DAPI is best used to preserve signal strength across prolonged imaging sessions of fluorescent dyes or fluorescent proteins during confocal laser scanning or with super resolution microscopy.

Features of ProLong Diamond Antifade Mountant with DAPI
ProLong Diamond Antifade Mountant with DAPI comes mixed and ready-to-use in a 2 mL dropper bottle or 10 mL bottle. When bound to DNA, DAPI can be detected using a traditional DAPI filter under UV light with an excitation of 360 nm and emission at 460 nm. ProLong Diamond Mountant with DAPI forms an optical path of 1.47 refractive index (RI) when cured and enables longer-term storage of your samples while protecting them from photobleaching. Just apply a drop to the sample, add a coverslip, cure, and image. ProLong Diamond Mountant with DAPI does not discolor or shrink when cured. With normal staining protocols, DAPI diffuses out of samples over time. The inclusion of DAPI within the Prolong Diamond mounting media formulation helps retain nuclear staining for long term storage compared to sequential staining/mounting in base mountant.

Get Photobleaching Protection Across the Entire Visible and IR Spectrums
No matter which fluorescent dyes or proteins you are using, ProLong Diamond mountant with DAPI helps provide superior protection against photobleaching. Compatible with a wide array of fluorescent dyes including Alexa Fluor dyes, FITC, TRTC, Texas Red, Cy3, and Cy5, our antifade mounting media with DAPI makes multiplexing smooth and effortless. ProLong Diamond Mountant with DAPI does not quench fluorophores, and its ability to provide brighter signal, against little background, helps ensure that you get the best quality, high-resolution images.

Understanding how RI impacts image resolution in microscopy
The RI determines how much the path of light is bent, or refracted, when entering a material. In microscopy, refractive index differences between specimen and mounting medium should be minimized and can be reduced by using mountants with a higher RI. Optimizing the RI of your experiment will increase the resolution to provide better quality images. Depending on your sample type, thickness, imaging depth and/or staining reagents (e.g. fluorescent proteins or dyes) and whether you need a curing or non-curing mountant, choosing the right mounting media will impact image resolution and should be strongly considered for optimal experimental design. Additionally, the curing property of ProLong Diamond means you do not need to use traditional acrylic resins (i.e., fingernail polish) for mounting.

For immediate viewing of the sample, choose our non-curing mountant, SlowFade Diamond Antifade Mountant with DAPI. Please note that ProLong Glass mounting media is recommended for samples > 10 microns and all immersion oil imaging applications.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionProLong™ Diamond Antifade Mountant with DAPI, 5 x 2 mL
Quantity5 x 2 mL
Product LineProLong™
Product TypeAntifade Mountant
Reagent TypeMounting Solution, Antifade Solution
Volume (Metric)2 mL
Unit SizeEach
Contents & Storage
Contains 5 dropper bottles contaning 2 mL reagent each.

• Store at 2-8°C or frozen at -5 to -30°C.
• Protect from light.
• Stable for at least 6 months after receiving.

Frequently asked questions (FAQs)

What is the difference between ProLong and SlowFade antifade reagents?

Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

If I use ProLong Gold Antifade Mountant to mount my slides, should I seal the edges?

ProLong Gold Antifade Mountant hardens overnight at room temperature. For short-term storage (a couple of weeks) you do not need to seal the edges of the coverslip, and the sample should be stable. Beyond that time, though, some dye conjugates will have an off-rate into the medium, so cold storage is recommended. Sealing the edges will prevent long-term discoloration (golden color) from developing around the edges of the coverslip as the anti-oxidants oxidize.

The edges may be sealed with melted paraffin, VALAP (1:1:1 vaseline, lanolin, paraffin) or epoxy glue. Nail polish is not recommended as various components of nail polish may diffuse into the mountant and quench fluorescent dyes.

Find additional tips, troubleshooting help, and resources within our Cell Imaging Support Center.

Some antifade mounting media stay as liquid whereas others harden. What is the benefit of having one that hardens?

If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I mounted my cells in ProLong antifade mounting medium, but now I want to go back and re-label them. Is there a way I can unmount the coverslip after it has cured (hardened)?

Yes. Put the slide in a Coplin jar or beaker filled with warm (37oC) PBS buffer and let it sit, no agitation is required. The hardened ProLong mountant will swell and may slide off or be easily dislodged. If cells are adherent to the coverslip, make certain the coverslip side containing the cell or tissue sample does not land face down in the container or become scratched upon handling. Remove the coverslip, wash a couple of times, and proceed with re-staining and re-mounting in new ProLong mountant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using ProLong antifade mounting medium. Do I need to let it cure before imaging? Do I need to seal the edges of the coverslip?

You can image before it cures (hardens), and it will still slow photobleaching, but you have to let it cure overnight to get the best refractive index (resolution). There is no need to seal the edges. In fact, if you seal before it cures, it won't cure correctly. If you are archiving the slide for more than a month, though, seal the edges with resin, paraffin or VALAP (1:1:1 vaseline, lanolin, paraffin) after it cures or there may be slight discoloration along the edges over time.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.