ProLong Diamond Antifade Mountant with DAPI is a glycerol-based liquid mountant designed to provide unparalleled antifade protection for fluorescence across the entire visual and IR spectrums. You can use ProLong Diamond Mountant with DAPI with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background. Use ProLong Diamond for bright, high-definition fluorescent images with DAPI already embedded within the mounting media for nuclear counterstains. ProLong Diamond mountant with DAPI is best used to preserve signal strength across prolonged imaging sessions of fluorescent dyes or fluorescent proteins during confocal laser scanning or with super resolution microscopy.
Features of ProLong Diamond Antifade Mountant with DAPI
ProLong Diamond Antifade Mountant with DAPI comes mixed and ready-to-use in a 2 mL dropper bottle or 10 mL bottle. When bound to DNA, DAPI can be detected using a traditional DAPI filter under UV light with an excitation of 360 nm and emission at 460 nm. ProLong Diamond Mountant with DAPI forms an optical path of 1.47 refractive index (RI) when cured and enables longer-term storage of your samples while protecting them from photobleaching. Just apply a drop to the sample, add a coverslip, cure, and image. ProLong Diamond Mountant with DAPI does not discolor or shrink when cured. With normal staining protocols, DAPI diffuses out of samples over time. The inclusion of DAPI within the Prolong Diamond mounting media formulation helps retain nuclear staining for long term storage compared to sequential staining/mounting in base mountant.
Get Photobleaching Protection Across the Entire Visible and IR Spectrums
No matter which fluorescent dyes or proteins you are using, ProLong Diamond mountant with DAPI helps provide superior protection against photobleaching. Compatible with a wide array of fluorescent dyes including Alexa Fluor dyes, FITC, TRTC, Texas Red, Cy3, and Cy5, our antifade mounting media with DAPI makes multiplexing smooth and effortless. ProLong Diamond Mountant with DAPI does not quench fluorophores, and its ability to provide brighter signal, against little background, helps ensure that you get the best quality, high-resolution images.
Understanding how RI impacts image resolution in microscopy
The RI determines how much the path of light is bent, or refracted, when entering a material. In microscopy, refractive index differences between specimen and mounting medium should be minimized and can be reduced by using mountants with a higher RI. Optimizing the RI of your experiment will increase the resolution to provide better quality images. Depending on your sample type, thickness, imaging depth and/or staining reagents (e.g. fluorescent proteins or dyes) and whether you need a curing or non-curing mountant, choosing the right mounting media will impact image resolution and should be strongly considered for optimal experimental design. Additionally, the curing property of ProLong Diamond means you do not need to use traditional acrylic resins (i.e., fingernail polish) for mounting.
For immediate viewing of the sample, choose our non-curing mountant, SlowFade Diamond Antifade Mountant with DAPI. Please note that ProLong Glass mounting media is recommended for samples > 10 microns and all immersion oil imaging applications.