ProLong® Live Antifade Reagent, for live cell imaging

Catalog number: P36975

Molecular Probes™  Related applications: Cellular Imaging

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ProLong® Live Antifade Reagent helps prevent the loss of fluorescent signal due to photobleaching during live cell imaging. When dyes are illuminated, they can degrade (usually permanently), reducing the sample’s fluorescent intensity and leading to the creation of free radical singlet oxygen, an element that can further degrade neighboring dye molecules. ProLong® Live Antifade Reagent metabolizes this element, increasing the signal intensity and duration while keeping the background signal low. ProLong® Live reagent has little-to-no effect on cellular viability, proliferation, or other cellular functions in experiments up to 48 hours.

Features of ProLong® Live Antifade Reagent include:
• Photobleach protection in live cell experiments, for fluorophores such as GFP, RFP, Hoechst 33342, MitoTracker®, LysoTracker®, and CellTracker®dyes
• Little-to-no effect on cellular viability, proliferation, or other cellular functions
• Easy to use, just add to the imaging solution or complete cell culture media or buffer, incubate, and image

ProLong® Live Antifade Reagent contains enzymes from the plasma membrane of naturally occurring E. coli. These enzymes metabolize the elements that can cause photobleaching. They work in the extracellular space and are not known to enter cells, so intracellular functions are minimally affected by ProLong® Live Antifade Reagent. As shown in figure below, cells grown in the presence of ProLong® Live Antifade Reagent display minimal effects on cellular viability or proliferation. ProLong® Live Antifade Reagent provides photobleach protection for most fluorophores frequently used in live cell fluorescent imaging, such as GFP, RFP, Hoechst 33342, MitoTracker®, LysoTracker® and CellTracker® dyes. ProLong® Live Antifade Reagent can be added to any cell culture media or buffer that is suitable for fluorescent imaging.
For Research Use Only. Not for use in diagnostic procedures.


Reagent Type: Anti-Fade Solution
Product Size: 1 mL
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

Provided in 1 vial contaning 1 mL. Enough for 1000 assays (0.1 mL imaging solution/assay) or 200 assays (0.5 mL imaging solution/assay).
When stored at ≤−20°C product is stable for at least 6 months, with up to 4 freeze-thaw cycles.
When stored at 2–8°C product should be used within 30 days (a precipitant may form at this temperature).


Manuals & protocols

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