Correcting confocal acquisition to optimize imaging of fluorescence resonance energy transfer by sensitized emission.
Authorsvan Rheenen J, Langeslag M, Jalink K
JournalBiophys J
PubMed ID15041688
Imaging of fluorescence resonance energy transfer (FRET) between suitable fluorophores is increasingly being used to study cellular processes with high spatiotemporal resolution. The genetically encoded Cyan (CFP) and Yellow (YFP) variants of Green Fluorescent Protein have become the most popular donor and acceptor pair in cell biology. FRET between these ... More
In vivo mammalian brain imaging using one- and two-photon fluorescence microendoscopy.
AuthorsJung JC, Mehta AD, Aksay E, Stepnoski R, Schnitzer MJ
JournalJ Neurophysiol
PubMed ID15128753
'One of the major limitations in the current set of techniques available to neuroscientists is a dearth of methods for imaging individual cells deep within the brains of live animals. To overcome this limitation, we developed two forms of minimally invasive fluorescence microendoscopy and tested their abilities to image cells ... More
Interaction of Maf transcription factors with Pax-6 results in synergistic activation of the glucagon promoter.
AuthorsPlanque N, Leconte L, Coquelle FM, Benkhelifa S, Martin P, Felder-Schmittbuhl MP, Saule S
JournalJ Biol Chem
PubMed ID11457839
'In the endocrine pancreas, alpha-cell-specific expression of the glucagon gene is mediated by DNA-binding proteins that interact with the G1 proximal promoter element. Among these proteins, the paired domain transcription factor Pax-6 has been shown to bind to G1 and to transactivate glucagon gene expression. Close to the Pax-6-binding site, ... More
Quality assessment of confocal microscopy slide based systems: performance.
AuthorsZucker RM
JournalCytometry A
PubMed ID16807897
'BACKGROUND: All fluorescence slide-based cytometry detections systems basically include the following components: (1) an excitation light source, (2) intermediate optics, and (3) a detection device consisting of a CCD camera or a PMT. The optical principles employed is slide-based systems are similar to those of confocal microscopes (CLSM). METHODS: The ... More
Spatial organization of bacteriorhodopsin in model membranes. Light-induced mobility changes.
AuthorsKahya N, Wiersma DA, Poolman B, Hoekstra D
JournalJ Biol Chem
PubMed ID12167614
'Bacteriorhodopsin is a proton-transporting membrane protein in Halophilic archaea, and it is considered a prototype of membrane transporters and a model for G-protein-coupled receptors. Oligomerization of the protein has been reported, but it is unknown whether this feature is correlated with, for instance, light activation. Here, we have addressed this ... More
Targeting the variable surface of African trypanosomes with variant surface glycoprotein-specific, serum-stable RNA aptamers.
AuthorsLorger M, Engstler M, Homann M, Göringer HU
JournalEukaryot Cell
PubMed ID12582125
African trypanosomes cause sleeping sickness in humans and Nagana in cattle. The parasites multiply in the blood and escape the immune response of the infected host by antigenic variation. Antigenic variation is characterized by a periodic change of the parasite protein surface, which consists of a variant glycoprotein known as ... More
Direct gene transfer into rat articular cartilage by in vivo electroporation.
AuthorsGrossin L, Cournil-Henrionnet C, Mir LM, Liagre B, Dumas D, Etienne S, Guingamp C, Netter P, Gillet P
JournalFASEB J
PubMed ID12724342
To establish a system for efficient direct in vivo gene targeting into rat joint, we have evaluated a strategy of gene transfer by means of the delivery of external electric pulses (EP) to the knee after intra-articular injection of a reporter gene (GFP). Rats were killed at various times after ... More
ssDNA-dependent colocalization of adeno-associated virus Rep and herpes simplex virus ICP8 in nuclear replication domains.
AuthorsHeilbronn R, Engstler M, Weger S, Krahn A, Schetter C, Boshart M
JournalNucleic Acids Res
PubMed ID14576307
The subnuclear distribution of replication complex proteins is being recognized as an important factor for the control of DNA replication. Herpes simplex virus (HSV) single-strand (ss)DNA-binding protein, ICP8 (infected cell protein 8) accumulates in nuclear replication domains. ICP8 also serves as helper function for the replication of adeno-associated virus (AAV). ... More
Endocytosis of a glycosylphosphatidylinositol-anchored protein via clathrin-coated vesicles, sorting by default in endosomes, and exocytosis via RAB11-positive carriers.
AuthorsGrünfelder CG, Engstler M, Weise F, Schwarz H, Stierhof YD, Morgan GW, Field MC, Overath P
JournalMol Biol Cell
PubMed ID12802073
Recently, proteins linked to glycosylphosphatidylinositol (GPI) residues have received considerable attention both for their association with lipid microdomains and for their specific transport between cellular membranes. Basic features of trafficking of GPI-anchored proteins or glycolipids may be explored in flagellated protozoan parasites, which offer the advantage that their surface is ... More
The mucin Muc4 potentiates neuregulin signaling by increasing the cell-surface populations of ErbB2 and ErbB3.
AuthorsFunes M, Miller JK, Lai C, Carraway KL, Sweeney C
JournalJ Biol Chem
PubMed ID16690615
Mucins provide a protective barrier for epithelial surfaces, and their overexpression in tumors has been implicated in malignancy. We have previously demonstrated that Muc4, a transmembrane mucin that promotes tumor growth and metastasis, physically interacts with the ErbB2 receptor tyrosine kinase and augments receptor tyrosine phosphorylation in response to the ... More
Spinning disk confocal microscopy of live, intraerythrocytic malarial parasites. 2. Altered vacuolar volume regulation in drug resistant malaria.
In the previous paper [Gligorijevic, B., et al. (2006) Biochemistry 45, pp 12400-12410], we reported on a customized Nipkow spinning disk confocal microscopy (SDCM) system and its initial application to DIC imaging of hemozoin within live, synchronized, intraerythrocytic Plasmodium falciparum malarial parasites. In this paper, we probe the biogenesis as ... More
Short-term repeated corticosterone administration enhances glutamatergic but not GABAergic transmission in the rat motor cortex.
AuthorsKula J, Blasiak A, Czerw A, Tylko G, Sowa J, Hess G
JournalPflugers Arch
PubMed ID26696244
'It has been demonstrated that stress impairs performance of skilled reaching and walking tasks in rats due to the action of glucocorticoids involved in the stress response. Skilled reaching and walking are controlled by the primary motor cortex (M1); however, it is not known whether stress-related impairments in skilled motor ... More
Fast varifocal two-photon microendoscope for imaging neuronal activity in the deep brain.
AuthorsSato M, Motegi Y, Yagi S, Gengyo-Ando K, Ohkura M, Nakai J
JournalBiomed Opt Express
PubMed ID28966846
Fluorescence microendoscopy is becoming a promising approach for deep brain imaging, but the current technology for visualizing neurons on a single focal plane limits the experimental efficiency and the pursuit of three-dimensional functional neural circuit architectures. Here we present a novel fast varifocal two-photon microendoscope system equipped with a gradient ... More