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Invitrogen
AP2 alpha Polyclonal Antibody
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FIGURE: 1 / 6
AP2 alpha Antibody (PA5-17359) in ICC/IF
Immunofluorescence analysis AP2 alpha was performed using 70% confluent log phase SK-BR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with AP2 alpha Polyclonal Antibody (Product PA5-17359) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius ov... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-17359
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a sequence of human AP-2a
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
147 µg/mL
Amount
14.7 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
TFAP2A is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. TFAP2A is the only AP-2 protein required for early morphogenesis of the lens vesicle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activating enhancer-binding protein 2 alpha; activating enhancer-binding protein 2-alpha; activator protein 2; AP-2 transcription factor; AP2-alpha; DNA-binding protein (AP-2); ERF-1; FLJ51761; OTTHUMP00000214240; OTTHUMP00000214243; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); unnamed protein product
Gene Aliases: AP-2; Ap-2 (a); AP-2alpha; Ap2; AP2alpha; AP2TF; BOFS; Tcfap2a; TFAP2
UniProt ID: (Human) P05549, (Mouse) P34056
Entrez Gene ID: (Human) 7020, (Mouse) 21418, (Rat) 306862
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
sequence-specific DNA binding
transcription regulator inhibitor activity
sequence-specific double-stranded DNA binding
core promoter proximal region sequence-specific DNA binding
transcription coactivator activity
transcription corepressor activity
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
kidney development
neural tube closure
lens morphogenesis in camera-type eye
outflow tract morphogenesis
keratinocyte development
optic vesicle morphogenesis
optic cup structural organization
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
nervous system development
sensory organ development
sensory perception of sound
negative regulation of cell proliferation
embryonic pattern specification
embryonic body morphogenesis
positive regulation of gene expression
neural crest cell development
anterior neuropore closure
trigeminal nerve development
oculomotor nerve formation
forebrain neuron development
positive regulation of cell migration
positive regulation of bone mineralization
response to lipopolysaccharide
embryonic forelimb morphogenesis
forelimb morphogenesis
negative regulation of epidermal growth factor receptor signaling pathway
regulation of cell proliferation
inner ear morphogenesis
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
positive regulation of neuron apoptotic process
skin development
regulation of cell differentiation
regulation of neuron differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
sympathetic nervous system development
embryonic camera-type eye morphogenesis
embryonic cranial skeleton morphogenesis
skeletal system morphogenesis
epidermis morphogenesis
palate development
lens induction in camera-type eye
face morphogenesis
bone morphogenesis
eyelid development in camera-type eye
cornea development in camera-type eye
positive regulation of tooth mineralization
cellular response to iron ion
basement membrane organization
metanephric nephron development
negative regulation of reactive oxygen species metabolic process
retina layer formation
Schwann cell development
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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