AccuCheck Counting Beads

Catalog number: PCB100

Molecular Probes™  Related applications: Flow Cytometry

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Description

AccuCheck Counting Beads are an efficient single-platform method for absolute cell counts that combines the advantages of direct flow cytometric immunophenotyping with the use of two different fluorescent beads (A and B beads). These two fluorospheres are used as a double internal standard for blood volume calculation. A known volume of AccuCheck Counting Beads is added to the same known volume of stained blood in a lyse-no-wash technique. The beads are counted along with cells. Since the concentration of beads is known, the number of cells per microliter (the absolute count) is obtained by relating the number of cells counted to the total number of fluorescent bead events. The cell number is then multiplied by the number of total fluorospheres per unit of volume. As the AccuCheck Counting Beads system contains two different fluorospheres in a known proportion, we can first assure the accuracy of the assay by verifying the proportion of both types of beads.

View information about all flow cytometry cell counting beads.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bead Diameter: Bead A: 6.40 µmBead B: 6.36 µm
Number of Reactions: 100
Flow Cytometer Laser Lines: 488 and 633/635
Form: Solution
Format: Tube(s)
Product Size: 10 mL
Excitation (nm): 488
Calibration Type: Flow Cytometry Cell Counting Beads
Detection Method: Fluorescent
Diameter (Metric): 6.4 µm, 6.36 µm
For Use With (Equipment): Flow Cytometer
Shipping Condition: Wet Ice

Contents & storage

Contains 1 bottle of AccuCheck counting beads (10 mL).

Store in refrigerator (2–8°C).

Documents

Manuals & protocols

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