Thermo Scientific™

Pierce™ Premium Grade DSP (dithiobis(succinimidyl propionate))

Catalog number:  PG82081

Related applications:

Protein Biology

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Thermo Scientific Pierce Premium Grade DSP is our highest quality formulation of this popular amine-reactive, cleavable crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade DSP provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention, and (d) change control notification. DSP is dithiobis(succinimidyl propionate), an amine-reactive crosslinker having NHS-ester reactive ends and a cleavable disulfide bond in its spacer arm. DSP contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxy-succinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.

Features of Premium Grade DSP:

DSP—a thiol-cleavable, homobifunctional and amine-reactive crosslinking agent
High quality—identity and purity confirmed by several tests, including quantitative NMR
Product integrity—enhanced level of testing and characterization compared to standard grade
Lot retention—ample supply of past lots retained to ensure future process testing
Change management—Change Control Notification (CCN) service
Consistent manufacture—batch-specific manufacturing documentation review

• Chemical crosslinking of intracellular proteins prior to cell lysis and immunoprecipitation
• 'Fix' protein interactions to allow identification of weak or transient protein interactions
• Fix tissues for immunostaining without damaging RNA
• Protein crosslinking to create bioconjugates via single-step reactions
• Immobilize proteins onto amine-coated surfaces

Product References
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
DSP (dithiobis(succinimidyl propionate)), Lomant's Reagent
For Research Use Only. Not for use in diagnostic procedures.


Product Line: Pierce™
Crosslinker Type: Homobifunctional
Labeling Method: Chemical Labeling
Reactive Moiety: NHS Ester
Solubility: DMSO (Dimethylsulfoxide), DMF (Dimethylformamide)
Spacer: Cleavable, Long
Quantity: 1g
Sufficient For: 2.47L of typical 1X (1mM) reaction solution

Contents & storage

Store desiccated at 4°C. Shipped at ambient temperature.