Chromogenic Coliform Agar (ISO)

Enumerate and differentiate coliforms and Escherichia coli in water samples with Thermo Scientific™ Oxoid™ Chromogenic Coliform Agar (ISO) prepared media plates, in accordance with ISO 9308-1 guidelines for water quality analysis. Target organisms are easy to differentiate, E.coli forming dark blue/violet colonies and coliforms pink to red colonies. Complete detection within 24 hours, removing the need for subculturing.

Chromogenic Coliform Agar (ISO) plates are a fast, accurate, and efficient way to detect coliforms and E.coli during microbiological quality testing of drinking water, disinfected pool water, and treatment plant samples.

• Conforms to ISO 9308-1 guidelines
• Easily and clearly differentiates E. coli and coliforms
• Ready-to-use convenience of prepared media
• Tested in accordance with the methods described in ISO 7704:2023

The guideline, ISO 9308-1:2014 Enumeration of Escherichia coli and coliform bacteria –Part 1: Membrane filtration method for waters with low bacterial background flora, specifies a method based on membrane filtration, subsequent culture on a Chromogenic Coliform Agar medium, and calculation of the number of target organisms in the sample.

Chromogenic Coliform Agar (ISO) utilizes specific enzymes associated with general coliforms and E.coli for differentiation.

• The chromogen Salmon GAL, when cleaved by D-galactosidase, produces a pink to red coloration; this coloration and enzyme are associated with coliforms.
• E.coli contains D-galactosidase which cleaves Salmon GAL; however, it also contains D-glucuronidase, which cleaves the chromogen X-glucuronide. The combination of these chromogens produces a dark blue to violet coloration associated with E.coli.

Tergitol-7 within the medium inhibits Gram positive bacteria and Gram negative bacteria with exception of coliforms.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.