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View additional product information for PowerEase™ 300W Power Supply (230 VAC) - FAQs (PS0301)
23 product FAQs found
Yes, the PowerEase 300W Power Supply (230 VAC) (Cat. No. PS0301) as well as the PowerEase 90W Power Supply (Cat. No. PS0090) are both compatible with the Owl chambers; confirmed for the Owl EasyCast B2 Mini Gel Electrophoresis System (Cat. No. B2) and the Owl A2 Large Gel System, 2.3L, 37.5 x 32.5 x 10.5cm (L x W x H) (Cat. No. A2). Also, vice versa, Owl 300XL power supply + adapters can be used with the Mini Gel Tank (Cat. No. A25977), XCell SureLock Mini-Cell (Cat. No. EI0001) and XCell4 SureLock Midi-Cell (Cat. No. WR0100).
Find additional tips, troubleshooting help, and resources within our Protein Gel Electrophoresis Chambers, Power Supplies, and Accessories Support Center.
This is not really recommended, but it is always possible to increase run time by lowering the voltage of the run. In general, the relationships are linear - i.e., decreasing voltage by half will double the run time.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
If you are running the gel at constant voltage, you do not need to increase the voltage regardless of the thickness of the gel.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Using constant voltage allows the current and power to decrease during the run, providing a safety margin in case of a break in the system. Having lower power is a safety benefit and will also decrease the chances of experiencing an overheating of the gel. Further, the constant voltage setting does not need adjustment to account for differences in number or thickness of gels being run.
Find additional tips, troubleshooting help, and resources within ourProtein Gel 1D Electrophoresis Support Center.
During NativePAGE electrophoresis, it is common for the current to drop below 1 mA. The PowerEase 300W Power Supply will register this as a “No Load” error and automatically shut off, resulting in the stopping of the gel run. Since there is no way to disable/turn off the “Load Check” feature in the PowerEase 300W Power Supply, we do not recommend using it for Native-PAGE electrophoresis. Alternatively, the ZOOM Dual Power Supply is capable of disabling the “Load Check” feature and is our recommended power supply for NativePAGE applications.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
One extra fuse is supplied with the PowerEase 300W Power Supply. For detailed instructions on replacing the fuse, please refer to Page 19 of the manual.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are the scenarios for this error with likely causes and solutions:
- Voltage changes to current
- Current limit was set too low due to which the ceiling for current was hit and constant mode changed from voltage to current. Increase current limit to 3 A.
- Current changes to voltage
- Voltage was set to low due to which the ceiling for voltage was hit and constant mode changed from current to voltage. Increase voltage limit to 250 V.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are likely reasons why this might happen:
- Electrophoresis systems were added or removed during a run
- Buffer leaking in a connected system
- Excessive temperature increase
- Excessive buffer evaporation
- Loose connection in a connected system
- Current set too low
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
- Load exceeds 3 A. The output maximum on the power supply is 3 A, so make sure that it is not exceeded.
- Blown fuse in the power supply. Replace fuse following instructions on Page 19 of the manual.
- Incorrect input voltage. Check input voltage switch near power inlet.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
This is due to overheating of the power supply. Please do the following:
- Turn off power supply. Check for sufficient airflow around the power supply fan. After cooling down, restart the power supply by turning the Power switch to the on position.
- If you cannot restart the instrument, turn off the power, disconnect the power cord from the outlet, and contact Technical Service by sending an e-mail to techsupport@thermofisher.com.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
This is most likely due to a ground leak detected during the run. Check the electrophoresis system for improper grounding and restart the power supply by turning the Power switch off and on.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
This indicates that the circuit has been interrupted. Please check the following:
- Verify that the running buffer is correct.
- Verify that all cables are attached correctly.
- Turn the Power switch off and on again; restart application.
- If you cannot restart the instrument, turn off the power, disconnect the power cord from the outlet, and contact Technical Service by sending an e-mail to techsupport@thermofisher.com.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
- Electrophoresis leads are not connected to the power supply or to the electrophoresis unit(s), or there is a broken circuit in the electrophoresis cell. Check the connections to the power supply and on your electrophoresis cell to make sure the connection is intact; check condition of wires in electrophoresis unit. Close the circuit by reconnecting the cables. Press Start/Pause to restart the run.
- High resistance due to tape left on a pre-cast gel, incorrect buffer concentration, or incorrect buffer volumes in the electrophoresis cell. Correct the condition by making sure the tape is removed from the pre-cast gel, buffers are prepared correctly, and the recommended volume of buffer is added to the electrophoresis unit.
- Current has dropped below acceptable rating (10 mA). The power supply has a built-in “No Load Detection” feature that can detect when the current drops below the acceptable rating of 10 mA.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
- AC power cord is not connected. Check AC power cord connections at both ends. Use the correct cord.
- The fuse has blown. Replace the fuse.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
For detailed instructions on replacing the fuse on the PowerEase 300W Power Supply, please refer to Page 19 of the manual (https://tools.thermofisher.com/content/sfs/manuals/PowerEase300W_UG_man.pdf).
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The PowerEase 300W Power Supply requires no periodic maintenance with the exception of an occasional dry wipe-down of the instrument.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The PowerEase 300W Power Supply is capable of operating at limiting voltage, limiting current, or limiting power. However, we recommend operating it at limiting voltage for most applications as it provides the following advantages:
- For most electrophoresis methods, resistance increases throughout the run. Under limiting voltage, current and power decrease throughout the run, providing an improving margin of safety over time.
- The same voltage setting can be used regardless of the number or thickness of gels being electrophoresed.
Note: With the current limiting setting, the voltage will increase as resistance increases to satisfy Ohm's law (V=IR). If no voltage limit is set and a local fault condition occurs, such as a poor connection, very high local resistance may cause the voltage to increase to the maximum capacity of the power supply. This may lead to local overheating and damage to the electrophoresis cell or create unsafe conditions. When operating under constant current conditions, we recommend setting a voltage limit on the power supply at or slightly above the maximum expected voltage. Power is a function of voltage and current P=IV. For a typical gel run, the current will decrease causing the voltage to increase when run at constant power. The power limiting function may be used when running sequencing gels to remove the ammonium persulfate from the wells and to heat the gel to an optimal temperature for DNA separations.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
When using the PowerEase 300W Power Supply with 1-2 proteins gels or blots, we recommend using the Constant Voltage mode for optimal performance.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
For best results, we do not recommend using the PowerEase 300W Power Supply at its maximum electrical load limits. Variations in buffer conditions can result in exceeding the power supply's maximum voltage, current, or power output capacity and produce undesirable variations in performance.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The product specifications for the PowerEase 300W Power Supply are listed on Page 18 of the manual (https://tools.thermofisher.com/content/sfs/manuals/PowerEase300W_UG_man.pdf).
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The PowerEase 300W Power Supply includes the following components:
- PowerEase 300W Power Supply
- Extra fuse
- Power cord
Note: Please use the power cord approved for your country/region. North America customers should use the Type B power cord; Japanese customers should use the JIS 8303 power cord; Chinese customers should use the GB 15934-2008 power cord; Australia/New Zealand customers should use the Type I power cord.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The PowerEase 300W Power Supply accommodates the running and transferring of 8 Mini gels, or 16 Midi gels.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The PowerEase 300W Power Supply is a fully programmable, microprocessor-controlled power supply designed for high-throughput gel electrophoresis. It has a straight-forward, intuitive interphase and includes the following features:
- Constant voltage, current, or power settings
- Built-in timer for walk-away gel electrophoresis
- Up to 10 custom programs with 10 steps each
- Four sets of output jacks that are compatible with most electrophoresis devices
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.