Thermo Scientific™

SureTect™ Salmonella Species PCR Assay

 Related applications:
Industrial Microbiology

Detect Salmonella species in even the most challenging food types with the Thermo Scientific™ SureTect™ Salmonella Species PCR Assay which incorporates optimized primer and probe components for highly-specific detection of Salmonella species. The assay has been validated with a variety of matrices and a range of samples sizes and enrichments, following the AOAC or NF validated methods, to reduce the need for in-house validation prior to use. The internal amplification control confirms correct assay conditions have occurred and results can be relied on.

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The SureTect Salmonella Species PCR Assay has been extensively tested on over 20 different matrices from food manufacturing environments including meat, dairy products, fish, fruit and vegetables, and production surfaces. Specific samples tested during independent external validation include food types most commonly associated with contamination by Salmonella species and also those food types known to be particularly challenging to PCR detection, for example, ground beef, raw poultry, eggs, non-fat dried milk and ready-to-eat meals.


SureTect Lysis Reagent 1 Tubes
SureTect Lysis Tube Caps
SureTect Proteinase K
SureTect Salmonella PCR Tubes
SureTect PCR Caps
Instructions for use

Enriched samples are pipetted into pre-filled lysis tubes, along with Proteinase K, before incubation to lyse any bacterial cells present in the sample and release their DNA into the solution. The lysate is then loaded into the SureTect Salmonella PCR Tube to re-hydrate the preloaded PCR pellet which contains all of the necessary components and reagents for PCR, including a probe, primers and DNA template for the internal amplification control (IAC). There is no need to vortex or mix the sample. The PCR tubes are then sealed, loaded into the Thermo Scientific™ SureTect™ PikoReal™ 24-well Real-Time PCR Instrument or Applied Biosystems™ 7500 Fast 96-well Real-Time PCR instrument, and the run started. After completing the run, interpreted results are clearly displayed and can be reported, stored, printed or downloaded as required.

The SureTect System is a unique solution combining speed and performance in an easy-to-use, cost-effective platform—giving you results you can trust.

  • Rapid actions: Fast, optimized single enrichment step and automatic result interpretation
  • Efficient operations: Streamlined lysis and PCR protocols that allow for the detection of multiple targets in the same run
  • Convenience: Pre-filled lysis tubes and tableted PCR reagents to minimize the number of pipetting steps and hands-on time
  • Throughput flexibility: Continuous or batch processing with the SureTect PikoReal and Applied Biosystems 7500 Fast 96-well instruments
  • Peace of mind: Validated workflows supported by our dedicated and experienced technical team

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Discover the simplicity of SureTect for PCR food pathogen testing

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Manuals & protocols