Thermo Scientific™

SureTect™ Listeria Species PCR Assay

 Related applications:
Industrial Microbiology
Detect Listeria species in even the most challenging food types with the Thermo Scientific™ SureTect™ Listeria Species PCR Assay which incorporates optimized primer and probe components for highly-specific detection of Listeria species. The internal amplification control confirms correct assay conditions have occurred and results can be relied on. The assay is AOAC RI and NF certified.

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The SureTect Listeria Species PCR Assay has been extensively tested on a wide range of food matrices and samples from food manufacturing environments including cheese, produce, cooked meats and production surfaces. Specific samples tested during independent external validation include food types most commonly associated with contamination by Listeria species and also those food types known to be particularly challenging to PCR detection, for example, blue-veined cheeses, lettuce, ham and uncooked sausages.


  • SureTect Lysis Reagent 1 Tubes
  • SureTect Lysis Tube Caps
  • SureTect Lysis Reagent 2
  • SureTect Proteinase K
  • SureTect Listeria PCR Tubes
  • SureTect PCR Caps
  • Instructions for use

Enriched samples are simply pipetted into pre-filled Lysis Tubes, along with Proteinase K, before incubation to lyse any bacterial cells present in the sample and release their DNA into the solution. The lysate is then loaded into the SureTect Listeria PCR Tube to re-hydrate the preloaded PCR pellet which contains all of the necessary components and reagents for PCR, including a probe, primers and DNA template for the internal amplification control (IAC). There is no need to vortex or mix the sample. The PCR tubes are then sealed, loaded into the Thermo Scientiific™ SureTect™ PikoReal™ 24-well Real-Time PCR iInstrument or Applied Biosystems™ 7500 Fast 96-well Real-Time PCR instrument and the run started. After completing the run, interpreted results are clearly displayed and can be reported, stored, printed or downloaded as required.

The SureTect System is a unique solution combining speed and performance in an easy-to-use, cost-effective platform—giving you results you can trust.

  • Rapid actions: Fast, optimized single enrichment step and automatic result interpretation
  • Efficient operations: Streamlined lysis and PCR protocols that allow for the detection of multiple targets in the same run
  • Convenience: Pre-filled lysis tubes and tableted PCR reagents to minimize the number of pipetting steps and hands-on time
  • Throughput flexibility: Continuous or batch processing with the SureTect PikoReal and 96-well 7500 Fast instruments
  • Peace of mind: Validated workflows supported by our dedicated and experienced technical team

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Discover the simplicity of SureTect for PCR food pathogen testing

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Manuals & protocols