Thermo Scientific™

SureTect™ E. coli O157:H7 PCR Assay

 Related applications:
Industrial Microbiology
Detect E. coli O157:H7 in as little as nine hours with the highly-specific, real-time Thermo Scientific™ SureTect™ E. coli O157:H7 PCR Assay. The assay incorporates an optimized liquid lysis step, providing a quick and simple method for preparing the sample for rapid detection on the Thermo Scientific™ SureTect™ PikoReal™ or Applied Biosystems™ 7500 Fast Real-Time PCR instruments. The assay has been validated with a variety of matrices, samples sizes, and enrichment volumes, following the AOAC, USDA MLG or NF validated methods, to reduce the need for in-house validation prior to use.

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The SureTect E. coli O157:H7 PCR Assay has been extensively tested on a range of food matrices from food manufacturing environments, including samples of raw ground beef, raw beef trim, fruit juice and produce.

  • SureTect Lysis Reagent 1 Tubes
  • SureTect Lysis Tube Caps
  • SureTect Proteinase K
  • SureTect E. coli O157:H7 PCR Tubes
  • SureTect PCR Caps
  • Instructions for use
Enriched samples are pipetted into pre-filled lysis tubes, along with Proteinase K, before incubation, to lyse any bacterial cells present in the sample and release their DNA into the solution. The lysate is then loaded into the SureTect E. coli O157:H7 PCR tube to re-hydrate the pre-loaded PCR pellet which contains all of the necessary components and reagents for PCR, including a probe, primers and DNA template for the internal amplification control (IAC). There is no need to vortex or mix the sample. The PCR tubes are then sealed, loaded into the SureTect PikoReal 24-well Real-Time PCR iInstrument or Applied Biosystems 7500 Fast 96-well Real-Time PCR instrument, and the run started using the SureTect software. After completing the run, interpreted results are clearly displayed and can be reported, stored, printed or downloaded as required. The internal amplification control confirms correct assay conditions have occurred.

The SureTect System is an optimized solution combining speed and performance in an easy-to-use, cost-effective platform—giving you results you can trust.
  • Rapid actions: Fast, optimized single enrichment step and automatic result interpretation
  • Efficient operations: Streamlined lysis and PCR protocols that allow for the detection of multiple targets in the same run
  • Convenience: Pre-filled lysis tubes and tableted PCR reagents to minimize the number of pipetting steps and hands-on time
  • Throughput flexibility: Continuous or batch processing with the SureTect PikoReal 24-well and Applied Biosystems 7500 Fast 96-well instruments
  • Peace of mind: Validated workflows supported by our dedicated and experienced technical team
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Discover the simplicity of SureTect for PCR food pathogen testing

Achieve fast product release, improve operational efficiencies and help protect your brand by choosing a molecular solution with simple workflows for the top foodborne pathogens.

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Manuals & protocols