Thermo Scientific™
Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide
Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon bovine MBP Thr-97 derived peptide, containing a P-X-(S⁄T)-P consensus sequence, where X is any residue.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Assay: | Serine⁄Threonine Kinase Assay |
|---|---|
| Assay Entry: | Biochemical phospho-specific activity (no antibody) |
| Excitation/Emission: | 405⁄465, 535 |
| Label or Dye: | Methylcoumarin |
| No. of Reactions: | 800 Reactions |
| Product Line: | Z´-LYTE® |
| Readout: | End Point |
| References: | BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978) |
| Shipping Condition: | Dry Ice |
| Substrate Properties: | Peptide-Based Substrate |
| Target Entry: | MAPK13, MAP2K1, MAP2K6, MAPK1, MAP3K8, BRAF, MAP2K2, MAPK12, MAPK3, BRAF V599E |
| Target Enzyme: | Serine⁄Threonine Kinases |
| Technique: | FRET |
| Validated Kinases: | BRAF, MAPK1, MAPK3, MAPK12, MAPK13, MAP2K1, MAP2K2, MAP3K6, MAP2K8 |
| Source: | Bovine MBP, Thr-97 derived peptide S5, Consensus seq Pro-X-(Ser⁄Thr)-Pro (APRTPGGRR) |
| Format: | 384-well plate |
Contents & storage
Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
