Thermo Scientific™

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide

Catalog number: PV3176
Related applications: Protein Assays & Analysis
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Thermo Scientific™

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide

Catalog number: PV3176
Related applications: Protein Assays & Analysis
Technical SupportCustomer Service
Catalog Number
PV3176
Unit Size
800 x 20 µL assays
Price (USD)
Price: 690.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV3176800 x 20 µL assays
Price: 690.00
Your Price:
-
Product Overview
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FAQ
Citations & References
Additional Information
Recommendations
Recommendations
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon bovine MBP Thr-97 derived peptide, containing a P-X-(S⁄T)-P consensus sequence, where X is any residue.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line
Z ́-LYTE™
Assay
Serine⁄Threonine Kinase Assay
Assay Entry
Biochemical phospho-specific activity (no antibody)
Excitation/Emission
405/465, 535
Format
384-well Plate
For Use With (Application)
FRET
Label or Dye
Methylcoumarin
No. of Reactions
800 Reactions
Quantity
800 x 20 μL
Readout
End Point
References
BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
Shipping Condition
Dry Ice
Source
Bovine MBP, Thr-97 Derived Peptide S5, Consensus Seq Pro-X-(Ser⁄Thr)-Pro (APRTPGGRR)
Substrate Properties
Peptide-Based Substrate
Target Entry
MAPK13, MAP2K1, MAP2K6, MAPK1, MAP3K8, BRAF, MAP2K2, MAPK12, MAPK3, BRAF V599E
Target Enzyme
Serine⁄Threonine Kinases
Validated Kinases
BRAF, MAPK1, MAPK3, MAPK12, MAPK13, MAP2K1, MAP2K2, MAP3K6, MAP2K8

Contents & Storage

Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.