Search Thermo Fisher Scientific
Thermo Scientific™
Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide isRead more
Catalog number PV3176
Price (USD)/ Each
832.00
-
Quantity:
800 x 20 μL Assays
Price (USD)/ Each
832.00
Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide
Catalog numberPV3176
Price (USD)/ Each
832.00
-
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon bovine MBP Thr-97 derived peptide, containing a P-X-(S/T)-P consensus sequence, where X is any residue.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product LineZ ́-LYTE™
AssaySerine/Threonine Kinase Assay
Assay EntryBiochemical phospho-specific activity (no antibody)
Excitation/Emission405/465, 535 nm
Format384-well Plate
For Use With (Application)FRET
Label or DyeMethylcoumarin
No. of Reactions800 x 20 μL Reactions
Quantity800 x 20 μL Assays
ReadoutEnd Point
ReferencesBBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
Shipping ConditionDry Ice
SourceBovine MBP, Thr-97 Derived Peptide S5, Consensus Seq Pro-X-(Ser⁄Thr)-Pro (APRTPGGRR)
Substrate PropertiesPeptide-Based Substrate
Target EntryMAPK13, MAP2K1, MAP2K6, MAPK1, MAP3K8, BRAF, MAP2K2, MAPK12, MAPK3, BRAF V599E
Target EnzymeSerine/Threonine Kinases
Validated KinasesBRAF, MAPK1, MAPK3, MAPK12, MAPK13, MAP2K1, MAP2K2, MAP3K6, MAP2K8
Unit SizeEach
Contents & Storage
Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.