Assay: | Serine⁄Threonine Kinase Assay |
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Assay Entry: | Biochemical phospho-specific activity (no antibody) |
Excitation/Emission: | 405⁄465, 535 |
Label or Dye: | Methylcoumarin |
No. of Reactions: | 800 Reactions |
Product Line: | Z´-LYTE® |
Readout: | End Point |
References: | BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978) |
Shipping Condition: | Dry Ice |
Substrate Properties: | Peptide-Based Substrate |
Target Entry: | MAPK13, MAP2K1, MAP2K6, MAPK1, MAP3K8, BRAF, MAP2K2, MAPK12, MAPK3, BRAF V599E |
Target Enzyme: | Serine⁄Threonine Kinases |
Technique: | FRET |
Validated Kinases: | BRAF, MAPK1, MAPK3, MAPK12, MAPK13, MAP2K1, MAP2K2, MAP3K6, MAP2K8 |
Source: | Bovine MBP, Thr-97 derived peptide S5, Consensus seq Pro-X-(Ser⁄Thr)-Pro (APRTPGGRR) |
Format: | 384-well plate |