Z'-LYTE™ Kinase Assay Kit - Tyrosine 1 Peptide

Catalog number:  PV3190

Related applications:

Protein Assays and Analysis

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for SRC.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well plate
Assay Entry: Biochemical phospho-specific activity (no antibody)
Assay: Tyrosine Kinase Assay
Excitation/Emission: 405⁄465, 535
No. of Reactions: 800 Reactions
Source: Src Substrate (EEEIYGEFE)
References: Nature, 373, pp. 536-9 (1995)
Readout: End Point
Shipping Condition: Dry Ice
Substrate Properties: Peptide-Based Substrate
Target Entry: CSF1R, NTRK2, PTK6, IGF1R, MATK, NTRK3, ROS1, BMX, PTK2B, EPHA3, EPHB4, ERBB4, KDR, NTRK1, SRMS, FRK, ITK, BTK, FES, BLK1, EPHA5
Target Enzyme: Tyrosine Kinases
Technique: FRET
Validated Kinases: BLK1, BMX, BTK, CSF1R, EPHA3, EPHA5, EPHB4, ERBB4, FES, FRK, IGF1R, ITK, KDR, MATK, NTRK1, NTRK2, NTRK3, PTK2B, PTK6, ROS1, SRMS
Product Line: Z´-LYTE®
Label or Dye: Methylcoumarin

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.