Z'-LYTE™ Kinase Assay Kit - Tyrosine 2 Peptide

Catalog number:  PV3191

Related applications:

Protein Assays and Analysis

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for ABL.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well plate
Assay Entry: Biochemical phospho-specific activity (no antibody)
Assay: Tyrosine Kinase Assay
Excitation/Emission: 405⁄465, 535
No. of Reactions: 800 Reactions
Source: Abl Substrate (EAIYAAPF)
References: Nature, 373, pp 536-9 (1995)
Readout: End Point
Shipping Condition: Dry Ice
Substrate Properties: Peptide-Based Substrate
Target Entry: ABL1 G250E, ABL1 Y253F, RET Y791F, SYK, TYRO3, EPHB2, LYNA, MERTK, RET V804L, SRCN1, ABL1 E255K, EPHA4, FYN, RET, ABL1 T315I, FLT3, LCK, YES1, MET, ABL1, ABL2, FGR, HCK, EPHA1, EPHA8, EPHB3, LYNB, EPHB1, SRC, CSK
Target Enzyme: Tyrosine Kinases
Technique: FRET
Validated Kinases: ABL1, ABL2, CSK2, EPHA1, EPHA4, EPHA8, EPHB1, EPHB2, MERTK, MET, RET, SRC, SRCN1, SYK, TYRO3, YES1, EPHB3, FGR, FLT, FYN, HCK, LCK, LYNA, LYNB
Product Line: Z´-LYTE®
Label or Dye: Methylcoumarin

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.