Z'-LYTE® Kinase Assay Kit - Tyrosine 2 Peptide

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for ABL.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Type: Tyrosine Kinase Assay
Excitation⁄Emission (nm): 405⁄465, 535
Format: 384-well plate
Label or Dye: Methylcoumarin
Number of Reactions: 800 Reactions
Product Line: Z´-LYTE®
Product Size: 800 x 20 µL assays
Protein or Peptide Source: Abl Substrate (EAIYAAPF)
Readout: End Point
References: Nature, 373, pp 536-9 (1995)
Substrate Properties: Peptide-Based Substrate
Target Enzyme: Tyrosine Kinases
Technique: FRET
Validated Kinases: ABL1, ABL2, CSK2, EPHA1, EPHA4, EPHA8, EPHB1, EPHB2, EPHB3, FGR, FLT, FYN, HCK, LCK, LYNA, LYNB, MERTK, MET, RET, SRC, SRCN1, SYK, TYRO3, YES1
Shipping Condition: Dry Ice

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

Documents

Manuals & protocols