Z'-LYTE™ Kinase Assay Kit - Tyrosine 4 Peptide

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide contains an X-(E⁄D)-Y-(I⁄L⁄V) consensus sequence, where X is any residue.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well plate
Assay Entry: Biochemical phospho-specific activity (no antibody)
Assay: Tyrosine Kinase Assay
Excitation/Emission: 405⁄465, 535
No. of Reactions: 800 Reactions
Readout: End Point
Shipping Condition: Dry Ice
Research Category: Signal Transduction
Substrate Properties: Peptide-Based Substrate
Target Entry: PDGFRA T674I, PDGFRB, FGFR1, FGFR2, EGFR, FGFR4, FLT1, MUSK, JAK3, FLT4, FGFR3, INSRR, PDGFRA D842V, EPHA2, PDGFRA, PDGFRA V561D
Target Enzyme: Tyrosine Kinases
Technique: FRET
Validated Kinases: EGFR, EPHA2, FGFR, FLT1, FLT4, INSRR, JAK3, MUSK, PDGFRA, PDGFRB
Product Line: Z´-LYTE®
Label or Dye: Methylcoumarin

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.