Fluorescein-Crosstide Substrate

Back to top

Description

A general substrate for Ser/Thr kinases, the Crosstide peptide substrate is based upon GSK-3alpha/beta.

For use in combination with the LanthaScreen® Tb-pCrosstide Antibody (PV3564) to evaluate inhibitors in medium to high throughput screening applications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Amino Acid Sequence: Fluorescein- GRPRTSSFAEG
Assay Entry: Biochemical terbium phospho-specific activity
Detection Method: Fluorescent
Gene ID (Entrez): 10000, 10298, 207, 208, 25400, 51347, 5566, 56164, 57144, 6197, 6198, 8536
Label or Dye: FITC (Fluorescein)
Research Category: Signal Transduction
Shipping Condition: Dry Ice
Substrate: Fluorescein-Crosstide
Substrate Properties: Peptide-Based Substrate
Target Entry: PAK4, TAOK3, CAMK2D, CAMK2A, PAK1, PRKACA, AKT2, RPS6KB1, AKT3, AKT1, PAK7, RPS6KA3
Target Enzyme: Serine⁄Threonine Kinases
Validated Kinases: AKT1, AKT2, AKT3, CAMK2A, CAMK2D, PAK1, PAK4, PAK7, PRKACA, RPS6KB1, RPS6KA3, TAOK3
Substrate Type: Kinase Substrate
Molecular Weight: 1524 Da

Contents & storage

1 mg/mL Fluorescein-Crosstide substrates in 50 mM HEPES, pH 7.5. Store at -20°C and protect from light.