Thermo Scientific™

LanthaScreen™ Tb-IKBa pSer32 Antibody Kit

Catalog number:  PV3562

Related applications:

Protein Assays and Analysis

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Description

An essential component of the canonical NF-kB pathway, phosphorylation of IkappaBalpha at Ser32 by IKK preceedes the transactivation of NF-kappaB.

For use in combination with the Fluorescein-IKK peptide substrate (PV3507) to evaluate inhibitors in medium to high throughput screening applications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibody Conjugate: Lanthanide Chelates, Terbium (Tb)
Antibody Molecular Weight: 150000 Da
Assay Entry: Biochemical terbium phospho-specific activity
Excitation/Emission: 340⁄490, 520
Label Type: Lanthanide Chelates
Label or Dye: Tb (Terbium), FITC (Fluorescein)
Product Line: LanthaScreen®
Reactivity: Human
Reactivity abrv: Hu
Readout: End Point
Shipping Condition: Dry Ice
Target Entry: IKBKE, CHUK, GRK5, GRK6, TBK1, IKBKB
Target Enzyme: Serine⁄Threonine Kinases
Technique: TR-FRET
Validated Kinases: CHUK, GRK5, GRK6, IKBKB, IKBKE
Format: 384-well plate
Gene ID (Entrez): 1147, 2869, 3551

Contents & storage

Terbium-labeled anti-IkappaBalpha [pSer32] antibody in HEPES buffered saline. Store at -20°C.