Thermo Scientific™

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 12 Peptide

Catalog number:  PV3673

Related applications:

Protein Assays and Analysis

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon CDK7, containing Ser-164 and a (S⁄T)-P-X-(K⁄R) consensus sequence, where X is any residue.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay: Serine⁄Threonine Kinase Assay
Assay Entry: Biochemical phospho-specific activity (no antibody)
Excitation/Emission: 405⁄465, 535
Label or Dye: Methylcoumarin
No. of Reactions: 800 Reactions
Product Line: Z´-LYTE®
Readout: End Point
References: Mol. Cell. Biol. Vol 21. pp. 88-99 (2001)
Shipping Condition: Dry Ice
Substrate Properties: Peptide-Based Substrate
Target Entry: CDK1, CDK2, CDK5
Target Enzyme: Serine⁄Threonine Kinases
Technique: FRET
Validated Kinases: CDK1, CDK2, CDK5
Source: CDK7 (GLAKSFGSPNRAY(pT)H)
Format: 384-well plate

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.