To facilitate in-house assay development around multiple target classes and assay formats, Tb-labeled species-specific secondary antibodies are available within the LanthaScreen® Toolbox of assay reagents. Tb-labeled secondary antibodies bind to their corresponding species IgG with low nanomolar apparent Kd's, and show negligible cross reactivity with IgG's from other species. Each Tb-labeled anti-species antibody is provided with a corresponding fluorescein-labeled IgG as a positive control.
In a kinase assay utilizing the Tb-labeled antibody generic reagent, anti-phosphopeptide primary antibody is not labeled and the detection step is performed by the addition of the Tb-labeled secondary antibody. This approach can be applied to other target classes and assay formats.
For Research Use Only. Not for use in diagnostic procedures.
Lanthanide Chelates, Terbium (Tb)
Contents & Storage
Store in freezer (-5 to -30°C).
Figure 1 - Titration of Fluorescein-Mouse IgG with 2 nM Tb-anti-Mouse Antibody
Figure 1 - Secondary antibody applications to kinase assays
Figure 1 - Titration of Fluorescein-Human IgG with 2 nM Tb-anti-Human Antibody
Figure 1 - Titration of Fluorescein-Rabbit IgG with 2 nM Tb-anti-Rabbit Antibody
Figure 1 - Titration of Fluorescein-Goat IgG with 2 nM Tb-anti-Goat Antibody