A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon myosin light chain 2, containing Ser-20 and a Hyd-X-R-X-X-(S⁄T) consensus sequence, where Hyd is a hydrophobic residue and X is any residue.
For Research Use Only. Not for use in diagnostic procedures.
For Use With (Application)
Serine/Threonine Kinase Assay
Biochemical phospho-specific activity (no antibody)
Label or Dye
Kinase Assay Kit
800 x 20 µL Assays
myosin light chain 2 (KKRPQRRYSNVF)
No. of Reactions
JBC(2001) 276, p38956
ROCK2, MYLK2, DAPK3, CDC42BPA, CDC42BPB, CAMK4
CAMK4, CDC42BPA, CDC42BPB, DAPK3, MYLK2, ROCK2
Contents & Storage
Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.