A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon myosin light chain 2, containing Ser-20 and a Hyd-X-R-X-X-(S⁄T) consensus sequence, where Hyd is a hydrophobic residue and X is any residue.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product Line
Z´-LYTE™
Target
Serine/Threonine Kinases
For Use With (Application)
FRET
Assay
Serine/Threonine Kinase Assay
Assay Entry
Biochemical phospho-specific activity (no antibody)
Label or Dye
Methylcoumarin
Product Type
Kinase Assay Kit
Quantity
800 x 20 µL Assays
Format
384-well plate
Sample Type
myosin light chain 2 (KKRPQRRYSNVF)
Excitation/Emission
405⁄465, 535
No. of Reactions
800 Reactions
References
JBC(2001) 276, p38956
Readout
End Point
Shipping Condition
Dry Ice
Substrate
Peptide-Based Substrate
Target Entry
ROCK2, MYLK2, DAPK3, CDC42BPA, CDC42BPB, CAMK4
Validated Kinases
CAMK4, CDC42BPA, CDC42BPB, DAPK3, MYLK2, ROCK2
Contents & Storage
Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.