Z'-LYTE™ Kinase Assay Kit - Ser/Thr 13 Peptide
Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon myosin light chain 2, containing Ser-20 and a Hyd-X-R-X-X-(S⁄T) consensus sequence, where Hyd is a hydrophobic residue and X is any residue.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Format: | 384-well plate |
|---|---|
| Assay Entry: | Biochemical phospho-specific activity (no antibody) |
| Assay: | Serine⁄Threonine Kinase Assay |
| Excitation/Emission: | 405⁄465, 535 |
| No. of Reactions: | 800 Reactions |
| Source: | myosin light chain 2 (KKRPQRRYSNVF) |
| References: | JBC(2001) 276, p38956 |
| Readout: | End Point |
| Shipping Condition: | Dry Ice |
| Substrate Properties: | Peptide-Based Substrate |
| Target Entry: | ROCK2, MYLK2, DAPK3, CDC42BPA, CDC42BPB, CAMK4 |
| Target Enzyme: | Serine⁄Threonine Kinases |
| Technique: | FRET |
| Validated Kinases: | CAMK4, CDC42BPA, CDC42BPB, DAPK3, MYLK2, ROCK2 |
| Product Line: | Z´-LYTE® |
| Label or Dye: | Methylcoumarin |
Contents & storage
Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
