Search Thermo Fisher Scientific
Z'-LYTE™ Kinase Assay Kit - Ser/Thr 13 Peptide
Catalog number: PV3793
Related applications:Error loading your content!
| Catalog number |
Select a plan
Unit size
|
Price ({{currency}}) | Availability | Qty | |
|---|---|---|---|---|---|
| {{product.sku}} {{product.sku}} also known as {{product.formattedSku}} |
|
— |
Pro add-ons
Your on-site stock
››
{{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
››
{{supplyCenter.scName}}(Out of stock)
››
{{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon myosin light chain 2, containing Ser-20 and a Hyd-X-R-X-X-(S⁄T) consensus sequence, where Hyd is a hydrophobic residue and X is any residue.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Specifications
| Assay Type: | Serine⁄Threonine Kinase Assay |
|---|---|
| Excitation⁄Emission (nm): | 405⁄465, 535 |
| Format: | 384-well plate |
| Label or Dye: | Methylcoumarin |
| Number of Reactions: | 800 Reactions |
| Product Line: | Z´-LYTE® |
| Product Size: | 800 x 20 µL assays |
| Protein or Peptide Source: | myosin light chain 2 (KKRPQRRYSNVF) |
| Readout: | End Point |
| References: | JBC(2001) 276, p38956 |
| Substrate Properties: | Peptide-Based Substrate |
| Target Enzyme: | Serine⁄Threonine Kinases |
| Technique: | FRET |
| Validated Kinases: | CAMK4, CDC42BPA, CDC42BPB, DAPK3, MYLK2, ROCK2 |
| Shipping Condition: | Dry Ice |
Contents & storage
Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
