Z'-LYTE™ Kinase Assay Kit - Ser/Thr 13 Peptide

Catalog number:  PV3793

Related applications:

Protein Assays and Analysis

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon myosin light chain 2, containing Ser-20 and a Hyd-X-R-X-X-(S⁄T) consensus sequence, where Hyd is a hydrophobic residue and X is any residue.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well plate
Assay Entry: Biochemical phospho-specific activity (no antibody)
Assay: Serine⁄Threonine Kinase Assay
Excitation/Emission: 405⁄465, 535
No. of Reactions: 800 Reactions
Source: myosin light chain 2 (KKRPQRRYSNVF)
References: JBC(2001) 276, p38956
Readout: End Point
Shipping Condition: Dry Ice
Substrate Properties: Peptide-Based Substrate
Target Entry: ROCK2, MYLK2, DAPK3, CDC42BPA, CDC42BPB, CAMK4
Target Enzyme: Serine⁄Threonine Kinases
Technique: FRET
Validated Kinases: CAMK4, CDC42BPA, CDC42BPB, DAPK3, MYLK2, ROCK2
Product Line: Z´-LYTE®
Label or Dye: Methylcoumarin

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.