Z'-LYTE® Kinase Assay Kit - Ser/Thr 13 Peptide

      Catalog number: PV3793

       Related applications: Pharma & Biopharma | Protein Assays and Analysis | Target & Lead Identification & Validation

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      Description

      A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon myosin light chain 2, containing Ser-20 and a Hyd-X-R-X-X-(S⁄T) consensus sequence, where Hyd is a hydrophobic residue and X is any residue.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Assay Type: Serine⁄Threonine Kinase Assay
      Excitation⁄Emission (nm): 405⁄465, 535
      Format: 384-well plate
      Label or Dye: Methylcoumarin
      Number of Reactions: 800 Reactions
      Product Line: Z´-LYTE®
      Product Size: 800 x 20 µL assays
      Protein or Peptide Source: myosin light chain 2 (KKRPQRRYSNVF)
      Readout: End Point
      References: JBC(2001) 276, p38956
      Substrate Properties: Peptide-Based Substrate
      Target Enzyme: Serine⁄Threonine Kinases
      Technique: FRET
      Validated Kinases: CAMK4, CDC42BPA, CDC42BPB, DAPK3, MYLK2, ROCK2
      Shipping Condition: Dry Ice

      Contents & storage

      Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

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      Manuals & protocols