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Thermo Scientific™

RAF1 (cRAF) [Y340D] [Y341D] Recombinant Human Protein

Catalog number:  PV4046

Related applications:

Protein Assays and Analysis

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Description

RAF1 (cRAF) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylates and activates the serine⁄threonine specific protein kinases ERK1 and ERK2. The RAF1 Y340D Y341D mutant is constitutively active.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NP_002871, Y340D, Y341D
Kinase Group: Ser⁄Thr Kinases: TKL Group
Shipping Condition: Dry Ice
Expression System: Baculovirus
Form: Frozen
Protein Form: Catalytic
Research Category: Drug Development
Species: Human
Validated Application: Kinase Assay
Molecular Weight: 66.4 kDa
Gene Alias: RAF1 (cRAF) Y340D Y341D
KinaseFamily: RAF Kinase Family
Protein Tag: GST Tag

Contents & storage

Storage Buffer: 50mM Tris, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% Triton X-100, 2mM DTT, 50% glycerol
Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.