A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for EGF Receptor.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product Line
Z´-LYTE™
Target
Tyrosine Kinases
For Use With (Application)
FRET
Assay
Tyrosine Kinase Assay
Assay Entry
Biochemical phospho-specific activity (no antibody)
Label or Dye
Methylcoumarin
Product Type
Kinase Assay Kit
Quantity
800 x 20 µL Assays
Format
384-well plate
Sample Type
EGFR substrate (EEEEYFELVAKKK)
Excitation/Emission
405⁄465, 535
No. of Reactions
800 Reactions
References
Nature, 373, pp. 536-9 (1995), JBC 277, pp 1576-1585 (2002)
Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.