Z'-LYTE™ Kinase Assay Kit - Tyrosine 6 Peptide
Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for EGF Receptor.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Format: | 384-well plate |
|---|---|
| Assay Entry: | Biochemical phospho-specific activity (no antibody) |
| Assay: | Tyrosine Kinase Assay |
| Excitation/Emission: | 405⁄465, 535 |
| No. of Reactions: | 800 Reactions |
| Source: | EGFR substrate (EEEEYFELVAKKK) |
| References: | Nature, 373, pp. 536-9 (1995), JBC 277, pp 1576-1585 (2002) |
| Readout: | End Point |
| Shipping Condition: | Dry Ice |
| Substrate Properties: | Peptide-Based Substrate |
| Target Entry: | KIT, JAK1, JAK2 JH1 JH2, JAK2 JH1 JH2 V617F, ERBB2, MST1R, LTK, JAK2, AXL |
| Target Enzyme: | Tyrosine Kinases |
| Technique: | FRET |
| Validated Kinases: | AXL, ERBB2, JAK1, JAK2, KIT, LTK, MST1R |
| Product Line: | Z´-LYTE® |
| Label or Dye: | Methylcoumarin |
Contents & storage
Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
