Z'-LYTE® Kinase Assay Kit - Tyrosine 6 Peptide

Catalog number: PV4122

 Related applications: Protein Assays and Analysis | Target & Lead Identification & Validation

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for EGF Receptor.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well plate
Readout: End Point
Technique: FRET
Assay Type: Tyrosine Kinase Assay
References: Nature, 373, pp. 536-9 (1995), JBC 277, pp 1576-1585 (2002)
Label or Dye: Methylcoumarin
Product Size: 800 x 20 µL assays
Target Enzyme: Tyrosine Kinases
Validated Kinases: AXL, ERBB2, JAK1, JAK2, KIT, LTK, MST1R
Number of Reactions: 800 Reactions
Substrate Properties: Peptide-Based Substrate
Protein or Peptide Source: EGFR substrate (EEEEYFELVAKKK)
Excitation⁄Emission (nm): 405⁄465, 535
Shipping Condition: Dry Ice

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

Documents

Manuals & protocols