Z'-LYTE™ Kinase Assay Kit - Tyrosine 6 Peptide

Catalog number:  PV4122

Related applications:

Protein Assays and Analysis

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for EGF Receptor.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well plate
Assay Entry: Biochemical phospho-specific activity (no antibody)
Assay: Tyrosine Kinase Assay
Excitation/Emission: 405⁄465, 535
No. of Reactions: 800 Reactions
Source: EGFR substrate (EEEEYFELVAKKK)
References: Nature, 373, pp. 536-9 (1995), JBC 277, pp 1576-1585 (2002)
Readout: End Point
Shipping Condition: Dry Ice
Substrate Properties: Peptide-Based Substrate
Target Entry: KIT, JAK1, JAK2 JH1 JH2, JAK2 JH1 JH2 V617F, ERBB2, MST1R, LTK, JAK2, AXL
Target Enzyme: Tyrosine Kinases
Technique: FRET
Validated Kinases: AXL, ERBB2, JAK1, JAK2, KIT, LTK, MST1R
Product Line: Z´-LYTE®
Label or Dye: Methylcoumarin

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.