Fluorescein-Ubiquitin

Name: Fluorescein-Ubiquitin
SKU: PV4378

Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due toMás información

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Número de catálogo	Cantidad
PV4378	500 μg
PV4377	50 μg

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Número de catálogo PV4378

Precio (MXN)

Cantidad:

500 μg

Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due to the method in labeling the ubiquitin protein, all of the lysines with the protein are available for further conjugation to form poly-ubiquitin chains.

For use in combination with FRET donors including LanthaScreen™ Tb-Ubiquitin (PV4375) or a LanthaScreen™ antibody including Tb-anti-GST (PV4216) or Tb-anti-ubiquitin-FK2 (PV4751).

For Research Use Only. Not for use in diagnostic procedures.

Especificaciones

Método de detecciónFluorescence

Excitación/emisión340⁄490, 520

Para utilizar con (equipo)Microplate Reader

Tipo de etiquetaClassic Dyes

Etiqueta o tinteFITC (Fluorescein)

Cantidad500 μg

Presentación de datosEnd Point

Condiciones de envíoDry Ice

Propiedades de sustratoProtein-Based Substrate

TécnicaTR-FRET

ConjugadoFluorescein

FormularioLiquid

Unit SizeEach

Contenido y almacenamiento

Fluorescein-labeled ubiquitin in HEPES buffered saline. Store at -20°C.

Citations & References (1)

Citations & References

Abstract

TR-FRET-based high-throughput screening assay for identification of UBC13 inhibitors.

Authors:Madiraju C, Welsh K, Cuddy MP, Godoi PH, Pass I, Ngo T, Vasile S, Sergienko EA, Diaz P, Matsuzawa S, Reed JC,

Journal:J Biomol Screen

PubMed ID:22034497

UBC13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBC13 is required for signaling by a variety of receptors important in immune regulation, ... More