Fluorescein-Ubiquitin

      Catalog number:  PV4378

       Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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      Description

      Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due to the method in labeling the ubiquitin protein, all of the lysines with the protein are available for further conjugation to form poly-ubiquitin chains.

      For use in combination with FRET donors including LanthaScreen® Tb-Ubiquitin (PV4375) or a LanthaScreen® antibody including Tb-anti-GST (PV4216) or Tb-anti-ubiquitin-FK2 (PV4751).
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Detection Method: Fluorescent
      Excitation⁄Emission (nm): 340⁄490, 520
      For Use With (Equipment): Microplate Reader
      Form: Frozen
      Label or Dye: FITC (Fluorescein)
      Product Size: 500 µg
      Readout: End Point
      Substrate Properties: Protein-Based Substrate
      Technique: TR-FRET
      Shipping Condition: Dry Ice

      Contents & storage

      Fluorescein-labeled ubiquitin in HEPES buffered saline. Store at -20°C.

      Documents

      Manuals & protocols