Fluorescein-Ubiquitin

Catalog number: PV4378

 Related applications: Target & Lead Identification & Validation

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Description

Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due to the method in labeling the ubiquitin protein, all of the lysines with the protein are available for further conjugation to form poly-ubiquitin chains.

For use in combination with FRET donors including LanthaScreen® Tb-Ubiquitin (PV4375) or a LanthaScreen® antibody including Tb-anti-GST (PV4216) or Tb-anti-ubiquitin-FK2 (PV4751).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Frozen
Readout: End Point
Technique: TR-FRET
Label or Dye: FITC (Fluorescein)
Product Size: 500 µg
Detection Method: Fluorescent
Substrate Properties: Protein-Based Substrate
For Use With (Equipment): Microplate Reader
Excitation⁄Emission (nm): 340⁄490, 520
Shipping Condition: Dry Ice

Contents & storage

Fluorescein-labeled ubiquitin in HEPES buffered saline. Store at -20°C.

Documents

Manuals & protocols