Phosphate Sensor

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The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ~ 0.1 µM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.
For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Format: 384-well plate
Readout: Kinetic
Technique: Fluorescence Intensity
Assay Type: Phosphatase Assay
Label or Dye: MDCC
Product Size: 100 nmol
Target Enzyme: GTPases, ATPases, Phosphatases, Phosphodiesterases
Detection Method: Fluorescent
For Use With (Equipment): Microplate Reader
Excitation⁄Emission (nm): 430⁄450
Shipping Condition: Dry Ice

Contents & storage

Phosphate Sensor is supplied as a 500 µM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C.


Manuals & protocols