Phosphate Sensor

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Description

The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ~ 0.1 µM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Type: Phosphatase Assay
Detection Method: Fluorescent
Excitation⁄Emission (nm): 430⁄450
For Use With (Equipment): Microplate Reader
Form: Liquid
Format: 384-well plate
Label or Dye: MDCC
Product Size: 100 nmol
Readout: Kinetic
Target Enzyme: ATPases, GTPases, Phosphatases, Phosphodiesterases
Technique: Fluorescence Intensity
Shipping Condition: Dry Ice

Contents & storage

Phosphate Sensor is supplied as a 500 µM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C.

Documents

Manuals & protocols