GFP-ATF2 (19-96)

Catalog number:  PV4445

Related applications:

Protein Assays and Analysis

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Description

A truncated version (residues 19-96) of ATF2 has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a substrate for the JNK and p38 families of kinases.

For use in combination with the LanthaScreen® Tb-anti-ATF2 [pThr71] antibody (PV4451) to evaluate inhibitors in medium to high throughput screening applications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry: Biochemical terbium phospho-specific activity
Detection Method: Fluorescent
Label or Dye: GFP (EmGFP)
Shipping Condition: Dry Ice
Substrate: ATF2 (19-96)
Substrate Properties: Protein-Based Substrate
Target Entry: MAPK13, MAPK8, MAPK9, MAPK14, MAPK3, MAPK1, MAPK11, MAPK12, MAPK10
Target Enzyme: Serine⁄Threonine Kinases
Validated Kinases: MAPK1, MAPK3, MAPK8, MAPK9, MAPK10, MAPK11, MAPK12, MAPK13, MAPK14
Substrate Type: Kinase Substrate
Molecular Weight: 478000 Da

Contents & storage

GFP-ATF2 protein substrate in 10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 1mM DTT. Store at -80°C.