A truncated version (residues 1-179) of c-Jun has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a substrate for the JNK family of kinases.
For use in combination with the LanthaScreen™ Tb-anti-c-Jun [pSer73] antibody (PV4453) to evaluate inhibitors in medium to high throughput screening applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assay Entry
Biochemical terbium phospho-specific activity
Detection Method
Fluorescent
Quantity
20 nmol
Substrate
c-Jun (1-179)
Substrate Properties
Protein-Based Substrate
Substrate Type
Kinase Substrate
Target Enzyme
Serine/Threonine Kinases
Label or Dye
GFP (EmGFP)
Molecular Weight (g/mol)
51.6 kDa
Shipping Condition
Dry Ice
Target Entry
NLK
Validated Kinases
MAPK8, MAPK9, MAPK10, NLK
Contents & Storage
GFP-c-Jun protein substrate in 10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 1 mM DTT. Store (at -80°C.)