Thermo Scientific™

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 21 Peptide

Catalog number:  PV4535

Related applications:

Protein Assays and Analysis

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This substrate is based upon CDC25C, containing Ser-216.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay: Serine⁄Threonine Kinase Assay
Assay Entry: Biochemical phospho-specific activity (no antibody)
Excitation/Emission: 405⁄465, 535
Label or Dye: Methylcoumarin
No. of Reactions: 800 Reactions
Product Line: Z´-LYTE®
Readout: End Point
Shipping Condition: Dry Ice
Substrate Properties: Peptide-Based Substrate
Target Entry: MARK1, MARK2, BRSK1
Target Enzyme: Serine⁄Threonine Kinases
Technique: FRET
Validated Kinases: BRSK1, MARK1, MARK2
Source: CDC25C residues 207-225, centered at Ser-216 (SRSGLYRSPSMPENLNRPRL)
Format: 384-well plate

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.