Thermo Scientific™

LanthaScreen™ Tb-p4E-BP1 (pThr46) Antibody Kit

Catalog number:  PV4757

Related applications:

Protein Assays and Analysis

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Description

A part of the PI3K/AKT Pathway, 4E-BP1 (EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1) is a cytosolic protein that is phosphorylated at Thr46 by mTORC1 upon stimulation of the pathway.

For use in combination with the GFP-4E-BP1 physiological protein substrate (PV4759) to evaluate inhibitors in medium to high throughput screening applications. An optimized mTor assay buffer (PV4794) is also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibody Conjugate: Lanthanide Chelates, Terbium (Tb)
Antibody Molecular Weight: 150000 Da
Assay Entry: Biochemical terbium phospho-specific activity
Excitation/Emission: 340⁄490, 520
Label Type: Lanthanide Chelates
Label or Dye: Tb (Terbium), GFP (EmGFP)
Product Line: LanthaScreen®
Reactivity: Human
Reactivity abrv: Hu
Readout: End Point
Shipping Condition: Dry Ice
Target Entry: mTOR, FRAP1
Target Enzyme: Serine⁄Threonine Kinases
Technique: TR-FRET
Validated Kinases: FRAP1 (mTOR), FRAP1 (mTOR)
Format: 384-well plate

Contents & storage

Terbium-labeled anti-4E-BP1 [pThr46] antibody in HEPES buffered saline. Store at -20°C.