A part of the PI3K/AKT Pathway, 4E-BP1 (EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1) is a cytosolic protein that is phosphorylated at Thr46 by mTORC1 upon stimulation of the pathway.
For use in combination with the GFP-4E-BP1 physiological protein substrate (PV4759) to evaluate inhibitors in medium to high throughput screening applications. An optimized mTor assay buffer (PV4794) is also available.
For Research Use Only. Not for use in diagnostic procedures.
Antibody Molecular Weight
Lanthanide Chelates, Terbium (Tb)
Biochemical terbium phospho-specific activity
Label or Dye
Tb (Terbium), GFP (EmGFP)
FRAP1 (mTOR), FRAP1 (mTOR)
Contents & Storage
Terbium-labeled anti-4E-BP1 [pThr46] antibody in HEPES buffered saline. Store at -20°C.
Figure 3 - Representative data for GFP-c-Jun (1-179) as a substrate for JNK1 kinase assays
Figure 4 - Representative data for GFP-4E-BP1 as a substrate for FRAP1 (mTOR) assay
Table 1 - Reactivity table for physiological protein substrates
Figure 2 - Representative data for GFP-ATF2 (19-96) as a substrate for JNK1 kinase assays
Figure 1 - LanthaScreen™ kinase assay using physiological protein substrates