GFP-EIF2A

Catalog number:  PV4809

Related applications:

Protein Assays and Analysis

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Description

EIF2A has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for the EIF2AK family of kinases including PERK (EIF2AK3) and PKR (EIF2AK2).

For use in combination with the LanthaScreen® Tb-anti-eIF2alpha [pSer52] Antibody (PV4810) to evaluate inhibitors in medium to high throughput screening applications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry: Biochemical terbium phospho-specific activity
Detection Method: Fluorescent
Gene ID (Entrez): 9833
Label or Dye: GFP (EmGFP)
Shipping Condition: Dry Ice
Substrate: EIF2A
Substrate Properties: Protein-Based Substrate
Target Entry: EIF2AK1, EIF2AK2, EIF2AK3
Target Enzyme: Serine⁄Threonine Kinases
Validated Kinases: EIF2AK2, EIF2AK3
Substrate Type: Kinase Substrate
Molecular Weight: 69100 Da

Contents & storage

GFP-EIF2alpha protein substrate in 9 mM HEPES pH 7.5, 123 mM NaCl, 2.43 mM KCl, 10% Glycerol, 2mM DTT . Store at -80 °C.