EIF2A has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for the EIF2AK family of kinases including PERK (EIF2AK3) and PKR (EIF2AK2).
For use in combination with the LanthaScreen™ Tb-anti-eIF2alpha [pSer52] Antibody (PV4810) to evaluate inhibitors in medium to high throughput screening applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assay Entry
Biochemical terbium phospho-specific activity
Detection Method
Fluorescent
Form
Liquid
Quantity
20 nmol
Substrate
EIF2A
Substrate Properties
Protein-Based Substrate
Substrate Type
Kinase Substrate
Target Enzyme
Serine/Threonine Kinases
Gene ID (Entrez)
9833
Label or Dye
GFP (EmGFP)
Molecular Weight (g/mol)
69100 Da
Shipping Condition
Dry Ice
Target Entry
EIF2AK1, EIF2AK2, EIF2AK3
Validated Kinases
EIF2AK2, EIF2AK3
Contents & Storage
GFP-EIF2alpha protein substrate in 9 mM HEPES pH 7.5, 123 mM NaCl, 2.43 mM KCl, 10% Glycerol, 2mM DTT . Store at -80 °C.