LanthaScreen™ Tb-peIF2a (pSer52) Antibody Kit

Catalog number:  PV4810

Related applications:

Protein Assays and Analysis

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Description

The alpha subunit of Eukaryotic initiation factor 2 (eIF2alpha) plays a key regulatory role in protein synthesis. In response to a cellular stress, phosphorylation of eIF2alpha at Ser52 occurs by the EIF2AK family of kinases including PERK (EIF2AK3) and PKR (EIF2AK2) to prohibit the initiation of protein synthesis.

For use in combination with the GFP-EIF2alpha physiological protein substrate (PV4809) to evaluate inhibitors in medium to high throughput screening applications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibody Conjugate: Lanthanide Chelates, Terbium (Tb)
Antibody Molecular Weight: 150000 Da
Antigen: Serine/threonine kinase
Assay Entry: Biochemical terbium phospho-specific activity
Excitation/Emission: 340⁄490, 520
Label Type: Lanthanide Chelates
Label or Dye: Tb (Terbium), GFP (EmGFP)
Product Line: LanthaScreen®
Reactivity: Human
Reactivity abrv: Hu
Readout: End Point
Shipping Condition: Dry Ice
Target Entry: EIF2AK1, EIF2AK2, EIF2AK3
Target Enzyme: Serine⁄Threonine Kinases
Technique: TR-FRET
Validated Kinases: EIF2AK2, EIF2AK3
Applications: Biochemical Kinase Assays
Conjugate: GFP (EmGFP), Tb (Terbium)
Format: 384-well plate
Gene Alias: HPK38, KIAA017
Quantity: 25μg
Regulatory Status: RUO
Storage Requirements: –20
Type: Primary
Primary or Secondary: Primary
Gene ID (Entrez): 9833
Packaging: 1 Each

Contents & storage

Terbium-labeled anti-eIF2alpha [pSer52] antibody in HEPES buffered saline. Store at -20°C.