Fluorescein-MAP2K1

Catalog number:  PV4812

Related applications:

Protein Assays and Analysis

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Description

Inactive MAP2K1 (MEK1) has been labeled with fluorescein, and is a substrate for MAPK family members.

For use in combination with the LanthaSreen™ Tb-MAP2K1 [pSer217/221] Antibody (PV4813) to evaluate inhibitors in medium to high throughput screening applications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry: Biochemical terbium phospho-specific activity
Detection Method: Fluorescent
Gene ID (Entrez): 10454, 10746, 1326, 4215, 4217, 4293, 4294, 4296, 5894, 673, 6885
Label or Dye: FITC (Fluorescein)
Shipping Condition: Dry Ice
Substrate: MAP2K1
Substrate Properties: Protein-Based Substrate
Target Entry: MAP3K2, RAF1 (cRAF) Y340D Y341D, MAP3K10, MAP3K9, MAP3K7/MAP3K7IP1, MAP3K5, MAP3K11, BRAF V599E, BRAF, MAP3K8, MAP3K3
Target Enzyme: Serine⁄Threonine Kinases
Validated Kinases: BRAF, MAP3K2, MAP3K3, MAP3K5, MAP3K7, MAP3K8, MAP3K9, MAP3K10, MAP3K11
Substrate Type: Kinase Substrate
Molecular Weight: 50000 Da

Contents & storage

Fluorescein-MAP2K1 protein substrate in 10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 1mM DTT. Store at -80°C.