LanthaScreen™ Tb-pMAP2K1 (pSer217/221) Antibody Kit

Catalog number:  PV4813

Related applications:

Protein Assays and Analysis

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Description

A member of the MAP Kinase family, MAP2K1 (mitogen-activated protein kinase kinase 1) is phosphorylated at Ser217/221 by a range of MAPK family members including Raf and B-Raf. For use in combination with the Fluorescein-MAP2K1 physiological protein substrate (PV4812) to evaluate inhibitors in medium to high throughput screening applications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibody Conjugate: Lanthanide Chelates, Terbium (Tb)
Antibody Molecular Weight: 150000 Da
Assay Entry: Biochemical terbium phospho-specific activity
Excitation/Emission: 340⁄490, 520
Label Type: Lanthanide Chelates
Label or Dye: Tb (Terbium), FITC (Fluorescein)
Reactivity: Human
Reactivity abrv: Hu
Readout: End Point
Shipping Condition: Dry Ice
Target Entry: MAP3K2, RAF1 (cRAF) Y340D Y341D, MAP3K10, MAP3K9, MAP3K7/MAP3K7IP1, MAP3K5, MAP3K11, BRAF V599E, BRAF, MAP3K8, MAP3K3
Target Enzyme: Serine⁄Threonine Kinases
Technique: TR-FRET
Validated Kinases: BRAF, MAP3K2, MAP3K3, MAP3K5, MAP3K7, MAP3K8, MAP3K9, MAP3K10, MAP3K11
Applications: Biochemical Kinase Assays
Conjugate: FITC (Fluorescein), Tb (Terbium), lanthanide chelates
Format: 384-well plate
Gene Alias: COT,EST,ESTF,FLJ10486,MEKK8,TPL2,Tpl-2,c-CO, CRAF,NS5,Raf-1,c-Ra, MEKK9,MLK1,PRKE, COT,EST,ESTF,FLJ10486,MEKK8,TPL2,Tpl-2,c-CO, MEKK7,TAK1,TGF1, MEKK9,MLK1,PRKE, ASK1,MAPKKK5,MEKK, MEKK10,MLK2,MS, MEKK11,MGC17114,MLK-3,MLK3,PTK1,SPR, CRAF,NS5,Raf-1,c-Ra,
Molecular Weight of Antigen: 150kDa
Quantity: 25μg
Regulatory Status: RUO
Storage Requirements: –20°C
Type: Primary
Primary or Secondary: Primary
Gene ID (Entrez): 10454, 10746, 1326, 4215, 4217, 4293, 4294, 4296, 5894, 673, 6885
Packaging: 1 Kit(s)
Includes: 0.02% Sodium azide

Contents & storage

Terbium-labeled anti-MAP2K1 [pSer217/221] antibody in HEPES buffered saline. Store at -20°C.