This kit contains a goat Tb-anti-GST antibody—an equivalent kit containing a rabbit-sourced antibody is also available.

LanthaScreen® TR-FRET PPAR alpha Competitive Binding Assay Kit, goat

Catalog number: PV4892

 Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15143:

The LanthaScreen® TR-FRET PPAR alpha Competitive Binding Assay provides a sensitive and robust method for high-throughput screening (HTS) of ligands for peroxisome proliferator-activated receptor alpha (PPAR alpha). The kit uses a terbium-labeled anti-GST antibody, a fluorescent small-molecule pan-PPAR ligand (Fluormone™ Pan-PPAR Green), and a human PPAR alpha ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen® TR-FRET PPAR alpha Competitive Binding Assay, Fluormone™ Pan-PPAR Green is added to ligand test compounds followed by addition of a mixture of the PPAR alpha-LBD and terbium anti-GST antibody. When the Fluormone™ Pan-PPAR Green is bound to PPAR alpha, energy transfer from the terbium-labeled antibody to the tracer occurs, and a high TR-FRET ratio is observed. Competitive ligand binding to PPAR alpha is detected by a test compound’s ability to displace the tracer, which results in a loss of FRET between the antibody and the tracer. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the IC50 from a dose response curve of the compound (Figure 1). This type of binding assay is analogous to radioligandbased assays, except that it eliminates handling of radioactivity and enables a homogeneous "addition-only" format.
For Research Use Only. Not for use in diagnostic procedures.


Format: 384-well plate
Readout: End Point
Technique: TR-FRET
Label or Dye: Tb (Terbium)
Product Size: 400 x 40 µL
Detection Method: Fluorescent
Validated Application: Competitive Binding Assay
For Use With (Equipment): Microplate Reader
Shipping Condition: Dry Ice

Contents & storage

The LanthaScreen® TR-FRET PPAR alpha Competitive Binding Assay Kit contains PPAR alpha-LBD (GST) protein, Fluormone™ Pan-PPAR Green, terbium-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).


Manuals & protocols

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