LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay Kit, goat

Catalog number: PV4892

LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay Kit, goat

Catalog number: PV4892
Catalog Number
PV4892
Unit Size
400 x 40 µL
Price (USD)
Price: 1,860.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV4892400 x 40 µL
Price: 1,860.00
Your Price:
-
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This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15143:

The LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay provides a sensitive and robust method for high-throughput screening (HTS) of ligands for peroxisome proliferator-activated receptor alpha (PPAR alpha). The kit uses a terbium-labeled anti-GST antibody, a fluorescent small-molecule pan-PPAR ligand (Fluormone™ Pan-PPAR Green), and a human PPAR alpha ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay, Fluormone™ Pan-PPAR Green is added to ligand test compounds followed by addition of a mixture of the PPAR alpha-LBD and terbium anti-GST antibody. When the Fluormone™ Pan-PPAR Green is bound to PPAR alpha, energy transfer from the terbium-labeled antibody to the tracer occurs, and a high TR-FRET ratio is observed. Competitive ligand binding to PPAR alpha is detected by a test compound’s ability to displace the tracer, which results in a loss of FRET between the antibody and the tracer. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the IC50 from a dose response curve of the compound (Figure 1). This type of binding assay is analogous to radioligandbased assays, except that it eliminates handling of radioactivity and enables a homogeneous “addition-only” format.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Conjugate
Tb (Terbium)
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Assay Entry
Biochemical competitive binding
Readout
End Point
Shipping Condition
Dry Ice
Target Entry
PPARA, PPAR alpha, NR1C1
Form
384-well Plate
Product Line
LanthaScreen™
For Use With (Application)
Competitive Binding Assay, TR-FRET
Ligand
Pan-PPAR
Product Type
TR-FRET PPAR Alpha Competitive Binding Assay Kit

Contents & Storage

The LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay Kit contains PPAR alpha-LBD (GST) protein, Fluormone™ Pan-PPAR Green, terbium-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).