LanthaScreen™ TR-FRET PPAR gamma Competitive Binding Assay Kit, goat

Catalog number: PV4894

LanthaScreen™ TR-FRET PPAR gamma Competitive Binding Assay Kit, goat

Catalog number: PV4894
Catalog Number
PV4894
Unit Size
400 x 40 µL
Price (USD)
Price: 1,858.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV4894400 x 40 µL
Price: 1,858.00
Your Price:
-
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This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15145:

The LanthaScreen™ TR-FRET PPAR gamma Competitive Binding Assay provides a sensitive and robust method for high-throughput screening (HTS) of ligands for peroxisome proliferator-activated receptor-gamma (PPAR gamma). The kit uses a terbium-labeled anti-GST antibody, a fluorescent small-molecule pan-PPAR ligand (Fluormone™ Pan-PPAR Green), and human PPAR gamma ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen™ TR-FRET PPAR gamma Competitive Binding Assay, Fluormone™ Pan-PPAR Green is added to ligand test compounds followed by addition of a mixture of the PPAR gamma-LBD and terbium anti-GST antibody. When the Fluormone™ Pan-PPAR Green is bound to PPAR gamma, energy transfer from the terbium-labeled antibody to the tracer occurs, and a high TR-FRET ratio is observed. Competitive ligand binding to PPAR gamma is detected by a test compound’s ability to displace the tracer, which results in a loss of FRET between the antibody and the tracer. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the IC50 from a dose response curve of the compound (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, “addition-only” format.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Conjugate
Tb (Terbium)
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Assay Entry
Biochemical competitive binding
Readout
End Point
Shipping Condition
Dry Ice
Target Entry
PPARG, PPAR gamma, NR1C3
Form
384-well Plate
Product Line
LanthaScreen™
For Use With (Application)
Competitive Binding Assay, TR-FRET
Ligand
Pan-PPAR
Product Type
TR-FRET PPAR Gamma Competitive Binding Assay Kit

Contents & Storage

The LanthaScreen™ TR-FRET PPAR gamma Competitive Binding Assay Kit contains PPAR gamma-LBD (GST) protein, Fluormone™ Pan-PPAR Green, terbium-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).