Fluorescein-ERM (LRRKtide)

Fluoresceína-ERM (LRRKtido)

Número de catálogo	Cantidad
PV4901	1 mg

Número de catálogo PV4901

Precio (CLP)

Cantidad:

A fluorescein-labeled peptide substrate based upon ezrin/radixin/moesin (ERM) that is phosphorylated by LRRK2 and LRRK2 mutants.

For use in combination with the LanthaScreen™ Tb-anti-ERM (pLRRKtide) Antibody (PV4899) to evalute inhibitors in medium to high throughput screening applications.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Secuencia de aminoácidosFluoresceína-GAGRLGRDKYKTLRQIRQ

Entrada del ensayoActividad bioquímica fosfoespecífica del terbio

ID de gen (Entrez)120892

Etiqueta o tinteFITC (fluoresceína)

Peso molecular2475,8 Da

Cantidad1 mg

Condiciones de envíoHielo seco

SustratoERM (LRRKtido)

Entrada dianaLRRK2 D1994A, LRRK2 R1441C, LRRK2 I2020T, LRRK2 G2019S, LRRK2

Quinasas validadasLRRK2

Método de detecciónFluorescente

Substrate PropertiesSustrato basado en péptidos

Tipo de sustratoSustrato de cinasa

Target EnzymeSerina/treonina cinasa

Unit SizeEach

Contenido y almacenamiento

400 µM de sustrato peptídico Fluoresceína-ERM (LRRKtido) en agua. Almacenar a -20 °C y proteger de la luz.

Citations & References (1)

Citations & References

Abstract

Investigation of leucine-rich repeat kinase 2 : enzymological properties and novel assays.

Authors:Anand VS, Reichling LJ, Lipinski K, Stochaj W, Duan W, Kelleher K, Pungaliya P, Brown EL, Reinhart PH, Somberg R, Hirst WD, Riddle SM, Steven P B,

Journal:FEBS J

PubMed ID:19076219

Mutations in leucine-rich repeat kinase 2 (LRRK2) comprise the leading cause of autosomal dominant Parkinson's disease, with age of onset and symptoms identical to those of idiopathic forms of the disorder. Several of these pathogenic mutations are thought to affect its kinase activity, so understanding the roles of LRRK2, and ... More