Citations & References (1)

Fluorescein-ERM (LRRKtide)

Catalog Number	Quantity
PV4901	1 mg

Catalog number PV4901

Price (USD)

1,184.00

Quantity:

Price (USD)

1,184.00

A fluorescein-labeled peptide substrate based upon ezrin/radixin/moesin (ERM) that is phosphorylated by LRRK2 and LRRK2 mutants.

For use in combination with the LanthaScreen™ Tb-anti-ERM (pLRRKtide) Antibody (PV4899) to evalute inhibitors in medium to high throughput screening applications.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Amino Acid SequenceFluorescein-GAGRLGRDKYKTLRQIRQ

Assay EntryBiochemical terbium phospho-specific activity

Gene ID (Entrez)120892

Label or DyeFITC (Fluorescein)

Molecular Weight (g/mol)2475.8 Da

Quantity1 mg

Shipping ConditionDry Ice

SubstrateERM (LRRKtide)

Target EntryLRRK2 D1994A, LRRK2 R1441C, LRRK2 I2020T, LRRK2 G2019S, LRRK2

Validated KinasesLRRK2

Detection MethodFluorescence

Substrate PropertiesPeptide-Based Substrate

Substrate TypeKinase Substrate

Target EnzymeSerine/Threonine Kinases

Unit SizeEach

Contents & Storage

400 μM Fluorescein-ERM (LRRKtide) peptide substrate in water. Store at -20°C and protect from light.

Citations & References (1)

Citations & References

Abstract

Investigation of leucine-rich repeat kinase 2 : enzymological properties and novel assays.

Authors:Anand VS, Reichling LJ, Lipinski K, Stochaj W, Duan W, Kelleher K, Pungaliya P, Brown EL, Reinhart PH, Somberg R, Hirst WD, Riddle SM, Steven P B,

Journal:FEBS J

PubMed ID:19076219

Mutations in leucine-rich repeat kinase 2 (LRRK2) comprise the leading cause of autosomal dominant Parkinson's disease, with age of onset and symptoms identical to those of idiopathic forms of the disorder. Several of these pathogenic mutations are thought to affect its kinase activity, so understanding the roles of LRRK2, and ... More