Specifically optimized for use with the Class I PI3 kinases (PIK3CA, PIK3CB, PIK3CD, and PIK3CG), this substrate consists of lipid vesicles of a 1:19 molar ratio of phosphoinositol-4,5-bisphosphate (PIP2) with phosphotidylserine (PS).
For use with the Adapta™ Universal Kinase Assay (PV5099).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assay Entry
Biochemical ADP accumulation
Detection Method
Fluorescent
Quantity
400 nmol
Substrate
Phosphoinositol-4,5-bisphosphate (PIP2) lipid vesicles with phosphotidylserine (PS)
Substrate Properties
Lipid-Based Substrate
Substrate Type
Kinase Substrate
Target Enzyme
Lipid Kinases
Gene ID (Entrez)
5290, 5294
Shipping Condition
Dry Ice
Target Entry
PIK3CG, PIK3CD/PIK3R1, PIK3CA/PIK3R1
Validated Kinases
PIK3CA, PIK3CG, PIK3CB, PIK3CD
Contents & Storage
PIP2:PS Lipid Kinase Substrate in 50 mM HEPES pH 7.5, 3 mM MgCl2, and 1 mM EGTA. Store at -20°C.