Predictor™ hERG Fluorescence Polarization Assay Kit

      Catalog number:  PV5365

       Related applications: Pharma & Biopharma | Target-Based ADME⁄Tox Assays

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      Description

      The Predictor™ hERG Fluorescence Polarization Assay Kit provides a set of validated components to perform hERG channel biochemical binding studies in the absence of radioligand. The assay was designed to identify potential hERG channel blockers by producing data that accurately correlates with patch-clamp electrophysiology studies. The assay is based on the principle of fluorescence polarization where a red-shifted fluorescent tracer is displaced from the hERG channel by compounds that bind to the channel. Assay performance was validated with a panel of established hERG channel blockers. Results from the Predictor™ assay demonstrate a high correlation with those obtained from patch clamp techniques.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Gene Symbol: hERG
      Protein Family: Ion Channels
      Color: Red
      Detection Method: Fluorescent
      Druggable Target: Ion Channels
      For Use With (Equipment): Microplate Reader
      Format: 384-well plate
      Label or Dye: Predictor™ hERG Tracer Red
      Product Line: Predictor™
      Product Size: 1 kit
      Readout: End Point
      System: Predictor™ System
      Shipping Condition: Dry Ice

      Contents & storage

      PV5365 Predictor™ hERG Fluorescence Polarization Assay Kit
      (Other materials are required separately; please refer to the protocol)
      Includes:
      • Predictor™ hERG Membranes
      • Predictor™ hERG Tracer Red
      • Predictor™ hERG Buffers
      • E-4031
      • Predictor™ hERG Fluorescence Polarization Assay Protocol
      • Certificate of Analysis
      Store the Predictor™ hERG Membranes at -80°C. The Predictor™ Tracer Red, Predictor™buffers, and E-4301 should be stored at -20°C.

      Documents

      Manuals & protocols