Predictor™ hERG Fluorescence Polarization Assay Kit

Catalog number:  PV5365

 Related applications:

Pharma & Biopharma

| Target-Based ADME⁄Tox Assays

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The Predictor™ hERG Fluorescence Polarization Assay Kit provides a set of validated components to perform hERG channel biochemical binding studies in the absence of radioligand. The assay was designed to identify potential hERG channel blockers by producing data that accurately correlates with patch-clamp electrophysiology studies. The assay is based on the principle of fluorescence polarization where a red-shifted fluorescent tracer is displaced from the hERG channel by compounds that bind to the channel. Assay performance was validated with a panel of established hERG channel blockers. Results from the Predictor™ assay demonstrate a high correlation with those obtained from patch clamp techniques.
For Research Use Only. Not for use in diagnostic procedures.


Gene Symbol: hERG
Protein Family: Ion Channels
Color: Red
Detection Method: Fluorescent
Druggable Target: Ion Channels
For Use With (Equipment): Microplate Reader
Format: 384-well plate
Label or Dye: Predictor™ hERG Tracer Red
Product Line: Predictor™
Product Size: 1 kit
Readout: End Point
System: Predictor™ System
Shipping Condition: Dry Ice

Contents & storage

PV5365 Predictor™ hERG Fluorescence Polarization Assay Kit
(Other materials are required separately; please refer to the protocol)
• Predictor™ hERG Membranes
• Predictor™ hERG Tracer Red
• Predictor™ hERG Buffers
• E-4031
• Predictor™ hERG Fluorescence Polarization Assay Protocol
• Certificate of Analysis
Store the Predictor™ hERG Membranes at -80°C. The Predictor™ Tracer Red, Predictor™buffers, and E-4301 should be stored at -20°C.


Manuals & protocols