Predictor™ hERG Fluorescence Polarization Assay Kit
Predictor™ hERG Fluorescence Polarization Assay Kit
인용 및 참조 문헌 (1)

Predictor™ hERG Fluorescence Polarization Assay Kit

Predictor™ hERG Fluorescence Polarization Assay Kit는 radioligand가 없는 상황에서 실시한 hERG channel biochemical binding 시험에 검증된 성분을 세트로 제공합니다. 이자세히 알아보기
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카탈로그 번호수량
PV53651 Kit
카탈로그 번호 PV5365
제품 가격(KRW)
1,699,000
온라인 행사
Ends: 31-Mar-2026
1,887,000
할인액 188,000 (10%)
Each
카트에 추가하기
수량:
1 Kit
제품 가격(KRW)
1,699,000
온라인 행사
Ends: 31-Mar-2026
1,887,000
할인액 188,000 (10%)
Each
카트에 추가하기
Predictor™ hERG Fluorescence Polarization Assay Kit는 radioligand가 없는 상황에서 실시한 hERG channel biochemical binding 시험에 검증된 성분을 세트로 제공합니다. 이 assay는 patch-clamp electrophysiology 시험과 정확하게 상관 관계가 있는 데이터를 생성하여 잠재적 hERG channel blocker를 파악하도록 설계되었습니다. 이는 fluorescence polarization 원리에 기초합니다. red-shifted fluorescent tracer가 hERG channel에 결합한 화합물로 이 채널에서 분리됩니다. Assay 성능은 입증된 hERG channel blocker 패널로 검증하였습니다. Predictor™ assay 결과는 patch clamp 기법의 결과와 큰 상관 관계를 보입니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
어세이 입력Biochemical binding
색상Red
검출 방법Fluorescence
약효를 기대할 수 있는 타겟Ion Channels
용도(장비)Microplate Reader
유전자 기호hERG
포장384-well Plate
제품 유형Assay Kit
단백질군Ion Channels
수량1 Kit
판독End Point
배송 조건Dry Ice
시스템 유형Predictor™ System
타겟 입력hERG
콘주게이트Predictor hERG Tracer Red
제품라인Predictor
Unit SizeEach
구성 및 보관

PV5365 Predictor™ hERG Fluorescence Polarization Assay Kit
(Other materials are required separately; please refer to the protocol)
Includes:
• Predictor™ hERG Membranes
• Predictor™ hERG Tracer Red
• Predictor™ hERG Buffers
• E-4031
• Predictor™ hERG Fluorescence Polarization Assay Protocol
• Certificate of Analysis
Store the Predictor™ hERG Membranes at -80°C. The Predictor™ Tracer Red, Predictor™buffers, and E-4301 should be stored at -20°C.

자주 묻는 질문(FAQ)

I am doing drug discovery. Can I use the Predictor hERG Membranes for radioligand binding?

No, the Predictor hERG Membranes are not suitable for radioligand binding.
These membranes contain extremely high levels of hERG channel protein. Radioligand binding assays on these membranes would require either abnormally high concentrations of radioligand or severe dilutions of the membrane preparation, which tend to have a deleterious effect on hERG channel conformation and stability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the common issues encountered with FP assays?

-No assay window: Check that your instrument is designed to do FP experiments.
-Poor discrimination between the minimum and maximum mP Control (both include the receptor): This may be an indication of the quality of the receptor. Do not make single use aliquots of the receptor or store diluted.
-The type of microplate is critical: Please see if the protocol specifies untreated polystyrene or NBS-coated plates. If the plate binds the fluorescent substrate, it cannot freely rotate and the mP reading will always be high. White plates cannot be used for FP assays. Avoid the use of silanized pipette tips and vials.
-The “No Receptor Control' (free Fluormone/Tracer) should have much higher RFU values compared to buffer alone. If not, there is a problem with the instrument setup or less commonly with the Fluormone/Tracer.
-If the free Fluormone/Tracer mP value is greater than 100 mP or negative, please refer to the user manual for your instrument and reset the G factor.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

What is the best type of microplate reader for fluorescence polarization (FP) assays?

For FP assays, plate readers must specifically have FP built in. Please see our instrument compatibility portal (http://www.thermofisher.com/us/en/home/industrial/pharma-biopharma/drug-discovery-development/dd-misc/instrument-compatibility-portal.html).

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

How can I test the fluorescence polarization (FP) settings of my microplate reader before doing the assay?

We offer a FP One-Step Reference Kit (https://www.thermofisher.com/order/catalog/product/P3088) to detect in green (Ex/Em = 488/535 nm) and red (Ex/Em = 525/590 nm) emission ranges, suitable for the PolarScreen Green and Red Assays. The alternative is to perform the Controls in the respective assay kit prior to performing the actual assay using test compounds.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Development of the predictor HERG fluorescence polarization assay using a membrane protein enrichment approach.
Authors:Piper DR, Duff SR, Eliason HC, Frazee WJ, Frey EA, Fuerstenau-Sharp M, Jachec C, Marks BD, Pollok BA, Shekhani MS, Thompson DV, Whitney P, Vogel KW, Hess SD,
Journal:Assay Drug Dev Technol
PubMed ID:18471075
The life-threatening consequences of acquired, or drug-induced, long QT syndrome due to block of the human ether-a-go-go-related gene (hERG) channel are well appreciated and have been the cause of several drugs being removed from the market in recent years because of patient death. In the last decade, the propensity for ... More