Kinase Tracer 199, 25 µM

Catalog number: PV5830

 Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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Description

In the LanthaScreen® Eu Kinase Binding Assay, binding of an Alexa Fluor® conjugate or "tracer" such as Kinase Tracer 199 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps. Visit www.invitrogen.com/bindingassay to learn more and to see which kinases have been validated using this kinase tracer.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Type: Kinase Binding Assay
Color: Far-Red
Detection Method: Fluorescent
Excitation⁄Emission (nm): 340⁄665
Label or Dye: Alexa Fluor™ 647
Product Size: 25 µL
Target Enzyme: Kinases (Multiple)
Technique: TR-FRET
Validated Kinases: EGFR T790M L858R, EPHB4, KIT V654A, MAPK9 (JNK2), RIPK2
Shipping Condition: Dry Ice

Contents & storage

Store at -20°C.

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Manuals & protocols

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