In the LanthaScreen® Eu Kinase Binding Assay, binding of an Alexa Fluor® conjugate or 'tracer' such as Kinase Tracer 199 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps. Visit www.invitrogen.com/bindingassay to learn more and to see which kinases have been validated using this kinase tracer.
For Research Use Only. Not for use in diagnostic procedures.