Thermo Scientific™

Kinase Tracer 222, 50 µM

Catalog number:  PV6121

Related applications:

Pharma & Biopharma

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Description

Kinase Tracer 222 is an Alexa Fluor™ 647 dye-labeled, ATP-competitive kinase inhibitor scaffold for use in the LanthaScreen™ Eu Kinase Binding Assay.

The LanthaScreen™ Eu Kinase Binding Assay
Is easily optimized
Measures inhibitor binding
Performs simple off-rate measurements

Easily Screen for Kinase Binding
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor™ conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (TR-FRET), while displacement of the tracer with a kinase inhibitor results in a loss of TR-FRET.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Assay: Kinase Binding Assay
Assay Entry: Biochemical binding/kinetic
Detection Method: Fluorescent
Excitation/Emission: 340/665
For Use With (Equipment): Microplate Reader
Readout: Kinetic
Shipping Condition: Dry Ice
Target Entry: STK33, NEK2, MAP3K7/MAP3K7IP1, CSNK1G1, IRAK4, HIPK3, CHEK2, CSNK1G2, CSNK1E, DAPK2
Target Enzyme: Kinases (Multiple)
Technique: TR-FRET
Color: Red
Label or Dye: Alexa Fluor™ 647
Product Line: LanthaScreen™
Format: 384-well plate

Contents & storage

Store at -20°C