Kinase Tracer 222, 50 µM

Catalog number: PV6121

 Related applications: Target & Lead Identification & Validation

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Kinase Tracer 222 is an Alexa Fluor® 647 dye-labeled, ATP-competitive kinase inhibitor scaffold for use in the LanthaScreen® Eu Kinase Binding Assay.

The LanthaScreen® Eu Kinase Binding Assay
Is easily optimized
Measures inhibitor binding
Performs simple off-rate measurements

Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor® conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (TR-FRET), while displacement of the tracer with a kinase inhibitor results in a loss of TR-FRET.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
•  Learn more about the LanthaScreen® Eu Binding Assay.

For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Color: Red
Format: 384-well plate
Readout: Kinetic
Technique: TR-FRET
Assay Type: Kinase Binding Assay
Label or Dye: Alexa Fluor™ 647
Product Size: 25 µL
Target Enzyme: Kinases (Multiple)
Detection Method: Fluorescent
For Use With (Equipment): Microplate Reader
Excitation⁄Emission (nm): 340⁄665
Shipping Condition: Dry Ice

Contents & storage

Store at -20°C

Documents

Manuals & protocols