Thermo Scientific™

Kinase Tracer 222, 50 µM

Catalog number: PV6121
Thermo Scientific™

Kinase Tracer 222, 50 µM

Catalog number: PV6121
Catalog Number
PV6121
Unit Size
25 µL
Price (USD)
Price: 502.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV612125 µL
Price: 502.00
Your Price:
-
Product Overview
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Kinase Tracer 222 is an Alexa Fluor™ 647 dye-labeled, ATP-competitive kinase inhibitor scaffold for use in the LanthaScreen™ Eu Kinase Binding Assay.

The LanthaScreen™ Eu Kinase Binding Assay
Is easily optimized
Measures inhibitor binding
Performs simple off-rate measurements

Easily Screen for Kinase Binding
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor™ conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (TR-FRET), while displacement of the tracer with a kinase inhibitor results in a loss of TR-FRET.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
384-well plate
For Use With (Application)
TR-FRET
Quantity
25 µL
Product Type
Kinase Tracer 222
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Readout
Kinetic
Shipping Condition
Dry Ice
Target
Kinases (Multiple)
Label or Dye
Alexa Fluor™ 647
Color
Red
Product Line
LanthaScreen™
Emission
665
Excitation
340

Contents & Storage

Store at -20°C