Kinase Tracer 222, 50 µM

Catalog number:  PV6121

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Pharma & Biopharma

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Kinase Tracer 222 is an Alexa Fluor® 647 dye-labeled, ATP-competitive kinase inhibitor scaffold for use in the LanthaScreen® Eu Kinase Binding Assay.

The LanthaScreen® Eu Kinase Binding Assay
Is easily optimized
Measures inhibitor binding
Performs simple off-rate measurements

Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor® conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (TR-FRET), while displacement of the tracer with a kinase inhibitor results in a loss of TR-FRET.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
•  Learn more about the LanthaScreen® Eu Binding Assay.

For Research Use Only. Not for use in diagnostic procedures.



Assay Type: Kinase Binding Assay
Color: Red
Detection Method: Fluorescent
Excitation⁄Emission (nm): 340⁄665
For Use With (Equipment): Microplate Reader
Format: 384-well plate
Label or Dye: Alexa Fluor™ 647
Product Line: LanthaScreen®
Product Size: 25 µL
Readout: Kinetic
Target Enzyme: Kinases (Multiple)
Technique: TR-FRET
Shipping Condition: Dry Ice

Contents & storage

Store at -20°C


Manuals & protocols