Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots.
AuthorsGiepmans BN, Deerinck TJ, Smarr BL, Jones YZ, Ellisman MH
JournalNat Methods
PubMed ID16179920
'The importance of locating proteins in their context within cells has been heightened recently by the accomplishments in molecular structure and systems biology. Although light microscopy (LM) has been extensively used for mapping protein localization, many studies require the additional resolution of the electron microscope. Here we report the application ... More
Analysis method for measuring submicroscopic distances with blinking quantum dots.
'A method is described that takes advantage of the intermittency ("blinking") in the fluorescence of quantum dots (QDs) to measure absolute positions of closely spaced QDs. The concept is that even if two QDs are separated by only tens of nanometers, the position of each QD is resolvable if the ... More
In vitro sliding of actin filaments labelled with single quantum dots.
AuthorsMånsson A, Sundberg M, Balaz M, Bunk R, Nicholls IA, Omling P, Tågerud S, Montelius L
JournalBiochem Biophys Res Commun
PubMed ID14733939
'We recently refined the in vitro motility assay for studies of actomyosin function to achieve rectified myosin induced sliding of actin filaments. This paves the way, both for detailed functional studies of actomyosin and for nanotechnological applications. In the latter applications it would be desirable to use actin filaments for ... More
Quantitative comparison of intracellular unpacking kinetics of polyplexes by a model constructed from quantum dot-FRET.
AuthorsChen HH, Ho YP, Jiang X, Mao HQ, Wang TH, Leong KW,
JournalMol Ther
PubMed ID18180773
'A major challenge for non-viral gene delivery is gaining a mechanistic understanding of the rate-limiting steps. A critical barrier in polyplex-mediated gene delivery is the timely unpacking of polyplexes within the target cell to liberate DNA for efficient gene transfer. In this study, the component plasmid DNA and polymeric gene ... More
DNA methylation detection using MS-qFRET, a quantum dot-based nanoassay.
AuthorsBailey VJ, Keeley BP, Razavi CR, Griffiths E, Carraway HE, Wang TH,
JournalMethods
PubMed ID20362674
'Detection of aberrant promoter hypermethylation of tumor suppressor genes can be used as a prognostic or predictive marker for carcinogenesis. Since epigenetic modifying agents are FDA approved for treatment of patients with myelodysplastic syndrome, laboratory correlative tools to monitor response to this targeted therapy are important. Methylation specific quantum dot ... More
In vivo multiphoton microscopy of deep brain tissue.
AuthorsLevene MJ, Dombeck DA, Kasischke KA, Molloy RP, Webb WW
JournalJ Neurophysiol
PubMed ID14668300
'Although fluorescence microscopy has proven to be one of the most powerful tools in biology, its application to the intact animal has been limited to imaging several hundred micrometers below the surface. The rest of the animal has eluded investigation at the microscopic level without excising tissue or performing extensive ... More
Application of quantum dots as probes for correlative fluorescence, conventional, and energy-filtered transmission electron microscopy.
AuthorsNisman R, Dellaire G, Ren Y, Li R, Bazett-Jones DP
JournalJ Histochem Cytochem
PubMed ID14688213
'Luminescent semiconductor quantum dots (QDs) are a new class of fluorescent label with wide-ranging applications for cell imaging. The electron density and elemental composition of these materials permit the extension of their use as probes in conventional electron microscopy (TEM) and energy-filtered TEM (EFTEM). Here we illustrate the feasibility of ... More
Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy.
AuthorsDoose S, Tsay JM, Pinaud F, Weiss S
JournalAnal Chem
PubMed ID15801758
'A number of different surface chemistries have been developed in recent years to render semiconductor nanocrystals (NCs) stable in water and biocompatible. However, most of these surface modifications affect NCs'' photophysical properties, calling for a method to simultaneously monitor colloidal and fluorescence properties. Fluorescence correlation spectroscopy (FCS) combined with ensemble ... More
Quantum dots as a visual aid for screening bead-bound combinatorial libraries.
AuthorsOlivos HJ, Bachhawat-Sikder K, Kodadek T
JournalChembiochem
PubMed ID14613120
Discrete nanostructures of quantum dots/Au with DNA.
AuthorsFu A, Micheel CM, Cha J, Chang H, Yang H, Alivisatos AP
JournalJ Am Chem Soc
PubMed ID15339154
Lighting up cells with quantum dots.
AuthorsWatson A, Wu X, Bruchez M
JournalBiotechniques
PubMed ID12613252
Quantum dots as a novel immunofluorescent detection system for Cryptosporidium parvum and Giardia lamblia.
AuthorsZhu L, Ang S, Liu WT
JournalAppl Environ Microbiol
PubMed ID14711692
Semiconductor quantum dot-conjugated antibodies were successfully developed to label Cryptosporidium parvum and Giardia lamblia. This novel fluorescence system exhibited superior photostability, gave 1.5- to 9-fold-higher signal-to-noise ratios than traditional organic dyes in detecting C. parvum, and allowed dual-color detection for C. parvum and G. lamblia. ... More
Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection.
AuthorsNess JM, Akhtar RS, Latham CB, Roth KA
JournalJ Histochem Cytochem
PubMed ID12871979
Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple ... More
Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking.
AuthorsDahan M, Lévi S, Luccardini C, Rostaing P, Riveau B, Triller A
JournalScience
PubMed ID14564008
Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the ... More
Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells.
In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Forster resonance energy transfer (FRET); and (5) spectral conversion of QDs exposed to high-intensity ... More
Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots.
AuthorsWu X, Liu H, Liu J, Haley KN, Treadway JA, Larson JP, Ge N, Peale F, Bruchez MP
JournalNat Biotechnol
PubMed ID12459735
Semiconductor quantum dots (QDs) are among the most promising emerging fluorescent labels for cellular imaging. However, it is unclear whether QDs, which are nanoparticles rather than small molecules, can specifically and effectively label molecular targets at a subcellular level. Here we have used QDs linked to immunoglobulin G (IgG) and ... More
Quantum dot biolabeling coupled with immunomagnetic separation for detection of Escherichia coli O157:H7.
AuthorsSu XL, Li Y
JournalAnal Chem
PubMed ID15307792
A sensitive, specific, and rapid method for the detection of E. coli O157:H7 was demonstrated using quantum dots (QDs) as a fluorescence marker coupled with immunomagnetic separation. Magnetic beads coated with anti-E. coli O157 antibodies were employed to selectively capture the target bacteria, and biotin-conjugated anti-E. coli antibodies were added ... More
Development and application of quantum dots for immunocytochemistry of human erythrocytes.
AuthorsTokumasu F, Dvorak J
JournalJ Microsc
PubMed ID12950474
Recent developments in quantum dot technology have resulted in the introduction of new fluorescence immunocytochemical probes. In contrast to organic fluorophores, which are not photostable, the high quantum yield and remarkable photostability of quantum dots solve major problems associated with immunocytochemical studies of erythrocytes. We report here the first application ... More
Detection of tumor marker CA125 in ovarian carcinoma using quantum dots.
AuthorsWang HZ, Wang HY, Liang RQ, Ruan KC
JournalActa Biochim Biophys Sin (Shanghai)
PubMed ID15483748
Semiconductor quantum dots (QDs) offer several advantages over organic dyes in fluorescence-imaging applications, such as higher quantum yield, exceptional photostability, and a narrow, tunable, and symmetric emission spectrum. To explore whether QDs could specifically and effectively label tumor markers and be used in immunohistochemistry as a novel type of fluorescent ... More
Single-quantum-dot-based DNA nanosensor.
AuthorsZhang CY, Yeh HC, Kuroki MT, Wang TH
JournalNat Mater
PubMed ID16379073
Rapid and highly sensitive detection of DNA is critical in diagnosing genetic diseases. Conventional approaches often rely on cumbersome, semi-quantitative amplification of target DNA to improve detection sensitivity. In addition, most DNA detection systems (microarrays, for example), regardless of their need for target amplification, require separation of unhybridized DNA strands ... More
Heat stress induced redistribution of fluorescent quantum dots in breast tumor cells.
AuthorsMinet O, Dressler C, Beuthan J
JournalJ Fluoresc
PubMed ID15615205
The probing of living cells in different colors over extended periods of time can be used to see the complicated processes that take place during carcinogenesis or heat stress, for example. Since most therapeutic laser tissue interactions are based on thermal effects a detailed characterization of thermal tissue damages in ... More
Visualization and quantitation of peroxisomes using fluorescent nanocrystals: treatment of rats and monkeys with fibrates and detection in the liver.
AuthorsColton HM, Falls JG, Ni H, Kwanyuen P, Creech D, McNeil E, Casey WM, Hamilton G, Cariello NF
JournalToxicol Sci
PubMed ID15084755
Peroxisome proliferation in the liver is a well-documented response that occurs in some species upon treatment with hypolipidemic drugs, such as fibrates. Typically, liver peroxisome proliferation has been estimated by direct counting via electron microscopy, as well as by gene expression, enzyme activity, and immunolabeling. We have developed a novel ... More
Use of semiconductor quantum dots for photostable immunofluorescence labeling of Cryptosporidium parvum.
AuthorsLee LY, Ong SL, Hu JY, Ng WJ, Feng Y, Tan X, Wong SW
JournalAppl Environ Microbiol
PubMed ID15466507
Cryptosporidium parvum is a waterborne pathogen that poses potential risk to drinking water consumers. The detection of Cryptosporidium oocysts, its transmissive stage, is used in the latest U.S. Environmental Protection Agency method 1622, which utilizes organic fluorophores such as fluorescein isothiocyanate (FITC) to label the oocysts by conjugation with anti-Cryptosporidium ... More