Qdot™ 800 Streptavidin Conjugate
Invitrogen™
Qdot™ 800 Streptavidin Conjugate
Qdot™ 800 streptavidin conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot™ nanocrystal). Streptavidin has aRead more
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Catalog NumberQuantity
Q10171MP200 μL
Q10173MP50 μL
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Catalog number Q10171MP
Price (USD)/ 200 µL
816.00
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Quantity:
200 μL
Price (USD)/ 200 µL
816.00
Add to cart
Qdot™ 800 Streptavidin Conjugate
Catalog numberQ10171MP
Price (USD)/ 200 µL
816.00
-
Add to cart
Qdot™ 800 streptavidin conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot™ nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot™ nanocrystal conjugates are provided as 1 μM solutions.

Important Features of Qdot™ Streptavidin Conjugates:
Streptavidin Qdot™ 800 conjugate has emission maxima of ∼800 nm
Approximately 5 to 10 streptavidins per Qdot™ nanocrystal
Extremely photostable and bright fluorescence
Efficiently excited with single-line excitation sources
Narrow emission, large Stokes shift
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Qdot™ Nanocrystals
The Qdot™ streptavidin conjugate is the size of a large macromolecule or protein (∼15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot™ streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot™ 705 and Qdot™ 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.

Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot™ colors or try the Qdot™ Streptavidin Sampler Kit, containing Qdot™ streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor™ dyes, Oregon Green™ dye, enzyme conjugates, and traditional fluorophores like Texas Red™ dye, fluorescein (FITC) and more.

Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

To learn more about Qdot™ Nanocrystals
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration1 μM
ConjugateQdot 800
Quantity200 μL
Shipping ConditionRoom Temperature
FormLiquid
Product LineQdot™
Product TypeStreptavidin Conjugate (fluorescent)
Unit Size200 µL
Contents & Storage
Store in refrigerator (2–8°C).