On-chip aptamer-based sandwich assay for thrombin detection employing magnetic beads and quantum dots.
Authors:Tennico YH, Hutanu D, Koesdjojo MT, Bartel CM, Remcho VT,
Journal:Anal Chem
PubMed ID:20545301
In this paper, we report the development of an on-chip aptamer-based fluorescence assay for protein detection and quantification based on sandwich ELISA principles. Thrombin was selected as a model analyte to validate the assay design, which involves two DNA thrombin aptamers recognizing two different epitopes of the protein. Aptamer-functionalized magnetic ... More