Qdot™ 605 Biotin Conjugate Kit

The biotin-labeled Qdot™ 605 nanocrystals are available for detecting streptavidin probes or for creating noncovalent conjugates with streptavidin-labeled molecules orRead more

Catalog Number	Quantity
Q10301MP	1 kit

Catalog number Q10301MP

Price (BRL)

Quantity:

1 kit

The biotin-labeled Qdot™ 605 nanocrystals are available for detecting streptavidin probes or for creating noncovalent conjugates with streptavidin-labeled molecules or with other biotinylated molecules using a streptavidin bridge. The product is provided as 250 μL of a 2 μM solution and includes 30 mL of Qdot™ incubation buffer.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product TypeBiotin Qdot Conjugation Kit

Quantity1 kit

Shipping ConditionRoom Temperature

Product LineQdot

Unit SizeEach

Contents & Storage

Store in refrigerator (2–8°C).

Frequently asked questions (FAQs)

What is the best way to remove white precipitate from my ITK Qdot nanocrystals?

Spinning your ITK Qdot nanocrystals at approximately 3,000 rpm for 3-5 minutes should remove the white precipitate from the supernatant. Use the supernatant immediately.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I see a white precipitate in my ITK Qdot nanocrystals; should I be concerned?

The precipitate in the organic ITK Qdot nanocrystals occurs with some frequency. The ITK Qdot nanocrystals sometimes include impurities that show as a white precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do my Qdot nanocrystals appear to be blinking?

Blinking is an inherent property of quantum dots; in fact, all single-luminescent molecules blink, including organic dyes. The brightness and photostability of Qdot nanocrystals makes the blinking more visibly apparent. Under higher energy excitation, Qdot nanocrystals blink even faster.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

My Qdot nanocrystals were brightly fluorescent before I mounted my samples; now I'm seeing a loss of fluorescence. Why is this happening?

Appropriate mounting media selection is very important to retain the fluorescence of Qdot nanocrystals. In our studies, Qdot nanocrystals work best with the following mountants:

HistoMount medium (Cat No. 00-8030); best for long term archiving
Cytoseal 60 Mountant
Clarion Mountant
Most polyvinyl alcohol-based mountants (limited storage time, less than weeks)
Water-based mountants (limited storage time, less than week)
Up to 50% glycerol (limited storage time, less than week)
Note: We do not recommend using ProLong mounting media with Qdot nanocrystals as it will quench their fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why can't I freeze my Qdot nanocrystal solution?

Freezing will cause the product to aggregate. The Qdot nanocrystals cannot be dispersed into solution after aggregation.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Qdot™ 605 Biotin Conjugate Kit FAQs

Citations & References (3)

Citations & References

Abstract

Nanoparticles as fluorescence labels: is size all that matters?

Authors:Swift JL, Cramb DT,

Journal:Biophys J

PubMed ID:18390610

'Fluorescent labels are often used in bioassays as a means to detect and characterize ligand-receptor binding. This is due in part to the inherently high sensitivity of fluorescence-based technology and the relative accessibility of the technique. There is often little concern raised as to whether or not the fluorescent label ... More

Measuring an antibody affinity distribution molecule by molecule.

Authors:Temirov JP, Bradbury AR, Werner JH,

Journal:Anal Chem

PubMed ID:18847284

Single molecule fluorescence microscopy was used to observe the binding and unbinding of hapten decorated quantum dots to individual surface immobilized antibodies. The fluorescence time history from an individual antibody site can be used to calculate its binding affinity. While quantum dot blinking occurs during these measurements, we describe a ... More

A two-photon excitation fluorescence cross-correlation assay for a model ligand-receptor binding system using quantum dots.

Authors:Swift JL, Heuff R, Cramb DT

Journal:Biophys J

PubMed ID:16299079

Two-photon excitation fluorescence cross-correlation spectroscopy (TPE-XCS) is a very suitable method for studying interactions of two distinctly labeled fluorescent molecules. As such, it lends itself nicely to the study of ligand-receptor interactions. By labeling the ligand with one color of fluorescent dye and the receptor with another, it is possible ... More