Human stem cell delivery for treatment of large segmental bone defects.
AuthorsDupont KM, Sharma K, Stevens HY, Boerckel JD, García AJ, Guldberg RE,
JournalProc Natl Acad Sci U S A
PubMed ID20133731
'Local or systemic stem cell delivery has the potential to promote repair of a variety of damaged or degenerated tissues. Although various stem cell sources have been investigated for bone repair, few comparative reports exist, and cellular distribution and viability postimplantation remain key issues. In this study, we quantified the ... More
Perturbational profiling of nanomaterial biologic activity.
'Our understanding of the biologic effects (including toxicity) of nanomaterials is incomplete. In vivo animal studies remain the gold standard; however, widespread testing remains impractical, and the development of in vitro assays that correlate with in vivo activity has proven challenging. Here, we demonstrate the feasibility of analyzing in vitro ... More
Immunomodulation of delayed-type hypersensitivity responses by mesenchymal stem cells is associated with bystander T cell apoptosis in the draining lymph node.
AuthorsLim JH, Kim JS, Yoon IH, Shin JS, Nam HY, Yang SH, Kim SJ, Park CG,
JournalJ Immunol
PubMed ID20802154
'Disease amelioration by mesenchymal stem cells (MSCs) has been shown to be closely related to their immunomodulatory functions on the host immune system in many disease models. However, the underlying mechanisms of how these cells affect the immune cells in vivo are not fully understood. In this study, we report ... More
Targeted tumor cell internalization and imaging of multifunctional quantum dot-conjugated immunoliposomes in vitro and in vivo.
AuthorsWeng KC, Noble CO, Papahadjopoulos-Sternberg B, Chen FF, Drummond DC, Kirpotin DB, Wang D, Hom YK, Hann B, Park JW,
JournalNano Lett
PubMed ID18712930
'Targeted drug delivery systems that combine imaging and therapeutic modalities in a single macromolecular construct may offer advantages in the development and application of nanomedicines. To incorporate the unique optical properties of luminescent quantum dots (QDs) into immunoliposomes for cancer diagnosis and treatment, we describe the synthesis, biophysical characterization, tumor ... More
Bioconjugation of the estrogen receptor hER(a) to a quantum dot dye for a controlled immobilization on a SiO(2) surface.
AuthorsCherkouk C, Rebohle L, Skorupa W,
JournalJ Colloid Interface Sci
PubMed ID21216405
'We investigated the immobilization of the estrogen receptor hER(a) on silanized SiO(2) surfaces for biosensor applications. The conjugation of the estrogen receptor hER(a) to the quantum dot dye QD655 was achieved. In order to obtain an optimal immobilization of the estrogen receptor hER(a) on the functionalized SiO(2) surface, the bioconjugate ... More
In vivo real-time, multicolor, quantum dot lymphatic imaging.
AuthorsKosaka N, Ogawa M, Sato N, Choyke PL, Kobayashi H,
JournalJ Invest Dermatol
PubMed ID19536144
'The lymphatic network is complex and difficult to visualize in real-time in vivo. Moreover, the direction of flow within lymphatic networks is often unpredictable especially in areas with well-developed ' ... More
Simultaneous multicolor imaging of five different lymphatic basins using quantum dots.
AuthorsKobayashi H, Hama Y, Koyama Y, Barrett T, Regino CA, Urano Y, Choyke PL
JournalNano Lett
PubMed ID17530812
Quantum dots can be used to perform multicolor images with high fluorescent intensity and are of a nanosize suitable for lymphatic imaging via direct interstitial injection. Here simultaneous multicolor in vivo wavelength-resolved spectral fluorescence lymphangiography is shown using five quantum dots with similar physical sizes but different emission spectra. This ... More
Sentinel lymph node imaging using quantum dots in mouse tumor models.
We demonstrate that quantum dots injected into two model tumors rapidly migrate to sentinel lymph nodes. PEG-coated quantum dots having terminal carboxyl, amino, or methoxyl groups all migrated from the tumor to surrounding lymph nodes similarly. Passage from the tumor through lymphatics to adjacent nodes could be visualized dynamically through ... More
microPET-based biodistribution of quantum dots in living mice.
This study evaluates the quantitative biodistribution of commercially available CdSe quantum dots (QD) in mice. (64)Cu-Labeled 800- or 525-nm emission wavelength QD (21- or 12-nm diameter), with or without 2,000 MW (molecular weight) polyethylene glycol (PEG), were injected intravenously into mice (5.55 MBq/25 pmol QD) and studied using well counting ... More