Targeted quantum dot conjugates for siRNA delivery.
AuthorsDerfus AM, Chen AA, Min DH, Ruoslahti E, Bhatia SN
JournalBioconjug Chem
PubMed ID17630789
'Treatment of human diseases such as cancer generally involves the sequential use of diagnostic tools and therapeutic modalities. Multifunctional platforms combining therapeutic and diagnostic imaging functions in a single vehicle promise to change this paradigm. in particular, nanoparticle-based multifunctional platforms offer the potential to improve the pharmacokinetics of drug formulations, ... More
Quantum dots are phagocytized by macrophages and colocalize with experimental gliomas.
AuthorsJackson H, Muhammad O, Daneshvar H, Nelms J, Popescu A, Vogelbaum MA, Bruchez M, Toms SA
JournalNeurosurgery
PubMed ID17327798
'OBJECTIVE: The identification of neoplastic tissue within normal brain during biopsy and tumor resection remains a problem in the operative management of gliomas. A variety of nanoparticles are phagocytized by macrophages in vivo. This feature may allow optical nanoparticles, such as quantum dots, to colocalize with brain tumors and serve ... More
In vivo imaging of quantum dots.
AuthorsTexier I, Josser V,
JournalMethods Mol Biol
PubMed ID19488714
'Noninvasive whole-body near-infrared fluorescence imaging is now acknowledged as a powerful method for the molecular mapping of biological events in live small animals such as mouse models. With outstanding optical properties such as high fluorescence quantum yields and low photobleaching rates, quantum dots (QDs) are labels of choice in the ... More
Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.
AuthorsLiu DS, Phipps WS, Loh KH, Howarth M, Ting AY,
JournalACS Nano
PubMed ID23181687
'We present a methodology for targeting quantum dots to specific proteins on living cells in two steps. In the first step, Escherichia coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13 amino acid recognition sequence that is genetically fused to a protein of interest. In the second ... More
Preparation of peptide-conjugated quantum dots for tumor vasculature-targeted imaging.
AuthorsCai W, Chen X,
JournalNat Protoc
PubMed ID18193025
'To take full advantage of the unique optical properties of quantum dots (QDs) and expedite future near-infrared fluorescence (NIRF) imaging applications, QDs need to be effectively, specifically and reliably directed to a specific organ or disease site after systemic administration. Recently, we reported the use of peptide-conjugated QDs for non-invasive ... More
Real-time imaging of astrocyte response to quantum dots: in vivo screening model system for biocompatibility of nanoparticles.
AuthorsMaysinger D, Behrendt M, Lalancette-Hébert M, Kriz J
JournalNano Lett
PubMed ID17638392
Astrocytes are the principle macroglial brain cells. They are activated by different stressors and brain injuries. Quantum dots (QDs) can cause oxidative stress. This study shows a real-time imaging of primary cortical cultures and assessment of QD-induced activation of astrocytes in the brains of transgenic mice with the luciferase gene ... More
Peptide-labeled near-infrared quantum dots for imaging tumor vasculature in living subjects.
AuthorsCai W, Shin DW, Chen K, Gheysens O, Cao Q, Wang SX, Gambhir SS, Chen X
JournalNano Lett
PubMed ID16608262
We report the in vivo targeting and imaging of tumor vasculature using arginine-glycine-aspartic acid (RGD) peptide-labeled quantum dots (QDs). Athymic nude mice bearing subcutaneous U87MG human glioblastoma tumors were administered QD705-RGD intravenously. The tumor fluorescence intensity reached maximum at 6 h postinjection with good contrast. The results reported here open ... More
Effect of poly(ethylene glycol) length on the in vivo behavior of coated quantum dots.
AuthorsDaou TJ, Li L, Reiss P, Josserand V, Texier I,
JournalLangmuir
PubMed ID19437711
The use of nanoparticles, either for the delivery of drugs or for imaging contrast agents, or a combination of both (theranostics), is very appealing in biological and biomedical research. The design of high-quality NIR-emitting quantum dots (QDs), with outstanding optical properties in comparison to that of organic dyes, should lead ... More